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Rockland
Cxcl1 Polyclonal Antibody
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Cxcl1 Antibody (600-401-MN2) in IHC
Immunohistochemistry of Rabbit Anti-Cxcl1 Antibody. Tissue: Mouse Lung. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20 mins. Primary Antibody: Anti-Cxcl1 at 1:100 for 30 mins at RT. Secondary Antibody: Anti-Rabbit Poly HRP-IgG Ready to Use for 8 mins at RT. Counter stain: Hematoxylin. Substrate: DAB. Results: shows moderate cytoplasmic staining in vascular endothelial cells, perivascular and peribronchiolar mononuclear cells and spindle cells (fibroblasts) at 1:100 dilution. There is intense cross reactivity wi... View More
Product Details
600-401-MN2
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Cxcl1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of mouse Cxcl1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Cxcl1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse.
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; CXCL-1; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51
Gene Aliases: Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1
UniProt ID: (Mouse) P12850
Entrez Gene ID: (Mouse) 14825
response to molecule of bacterial origin
acute inflammatory response
positive regulation of leukocyte chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of sodium ion transport
neutrophil chemotaxis
response to lipopolysaccharide
positive regulation of superoxide anion generation
positive regulation of potassium ion transport
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil mediated killing of fungus
positive regulation of hematopoietic stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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