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Invitrogen
Cyclin A Polyclonal Antibody
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FIGURE: 1 / 2
Cyclin A Antibody (PA5-109491) in WB
Western blot analysis of Cyclin A in extracts of various cell lines. Samples were incubated with Cyclin A Polyclonal antibody (Product # PA5-109491) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
PA5-109491
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CCNA1 (NP_001104515.1).
Conjugate
Form
Liquid
Concentration
0.95 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854902
Product Specific Information
Immunogen sequence: METGFPAIMY PGSFIGGWGE EYLSWEGPGL PDFVFQQPVE SEAMHCSNPK SGVVLATVAR GPDACQILTR APLGQDPPQR TVLGLLTANG QYRRTCGQGI TRIRCYSGSE NAFPPAGKKA LPDCGVQEPP KQGFDIYMDE LEQGDRDSCS VREGMAFEDV YEVDTGTLKS DLHFLLDFNT VSPMLVDSSL LSQSEDISSL GTDVINVTEY AEEIYQYLRE AEIRHRPKAH
Target Information
The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cyclin A; Cyclin-A; Cyclin-A1; Cyclin-A2; CyclinA; CyclinA1; testicular tissue protein Li 34
Gene Aliases: CCN1; CCNA; CCNA1; CCNA2; CT146
UniProt ID: (Human) P78396, (Human) P20248, (Rat) Q6AY13
Entrez Gene ID: (Human) 8900, (Human) 890, (Rat) 295052, (Rat) 114494
regulation of cyclin-dependent protein serine/threonine kinase activity
mitotic nuclear division
regulation of G2/M transition of mitotic cell cycle
cell division
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
male meiosis I
spermatogenesis
Ras protein signal transduction
organ regeneration
response to estradiol
response to glucagon
positive regulation of transcription, DNA-templated
positive regulation of fibroblast proliferation
mitotic G2 DNA damage checkpoint
viral process
cellular response to platelet-derived growth factor stimulus
cellular response to leptin stimulus
cellular response to cocaine
cellular response to luteinizing hormone stimulus
cellular response to estradiol stimulus
cellular response to hypoxia
cellular response to nitric oxide
cochlea development
cellular response to insulin-like growth factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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