Invitrogen

Cyclin A2 Monoclonal Antibody (E23.1)

Cyclin A2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

Cyclin A2 Antibody (MA1-154) in ICC/IF

Immunofluorescence analysis of Cyclin A2 was performed using 70% confluent log phase HeLa cells treated with Camptothecin (100nM, 16 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin A2 Monoclonal Antibody (Product # MA1-154) at 1:50 dilution in 0... View More

Cyclin A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cyclin A2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

MA1-154

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:50

Immunoprecipitation (IP)

1 µg

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

E23.1

Immunogen

Bovine Cyclin A fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536862

Product Specific Information

MA1-154 detects Cyclin A in mammalian samples and has been successfully used in Immunohistochemistry (Frozen and Paraffin), Immunofluorescence, Immunoprecipitation and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-154 show the accumulation of a prominent band at ~50 kDa in camptothecin and hydroxyurea treated cells. In Immunofluorescence applications, MA1-154 shows nuclear translocation of Cyclin A in G2/M arrested cells as a result of camptothecin treatment.

Target Information

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lysophosphatidic Acid Mediates Imiquimod-Induced Psoriasis-like Symptoms by Promoting Keratinocyte Proliferation through LPAR1/ROCK2/PI3K/AKT Signaling Pathway.

International journal of molecular sciences

Kim D,Kim HJ,Baek JO,Roh JY,Jun HS

Published figure using Cyclin A2 monoclonal antibody (Product # MA1-154) in Western Blot

Tue Oct 05 00:00:00 UTC 2021

Single-tubule RNA-Seq uncovers signaling mechanisms that defend against hyponatremia in SIADH.

Kidney international

Lee JW,Alsady M,Chou CL,de Groot T,Deen PMT,Knepper MA,Ecelbarger CM

MA1-154 was used in Western Blotting to identify signalling pathways activated at the onset of vasopressin-escape.

Mon Jan 01 00:00:00 UTC 2018

Survivin as a potential therapeutic target of acetylsalicylic acid in pituitary adenomas.

Oncotarget

Németh K,Szücs N,Czirják S,Reiniger L,Szabó B,Barna G,Karászi K,Igaz P,Zivkovic V,Korbonits M,Patócs A,Butz H

Published figure using Cyclin A2 monoclonal antibody (Product # MA1-154) in Western Blot

Tue Jun 26 00:00:00 UTC 2018

APOLLON protein promotes early mitotic CYCLIN A degradation independent of the spindle assembly checkpoint.

The Journal of biological chemistry

Kikuchi R,Ohata H,Ohoka N,Kawabata A,Naito M

MA1-154 was used in western blot to study the spindle assembly checkpoint-independent regulation of mitotic cyclin A by the APOLLON protein

Fri Feb 07 00:00:00 UTC 2014

Disrupting Skp2-cyclin A interaction with a blocking peptide induces selective cancer cell killing.

Molecular cancer therapeutics

Ji P,Sun D,Wang H,Bauzon F,Zhu L

MA1-154 was used in immunoprecipitation and western blot to report that the Skp2-cyclin A interaction has a major role in cancer cell survival

Thu Feb 01 00:00:00 UTC 2007

Differential apoptosis by gallotannin in human colon cancer cells with distinct p53 status.

Molecular carcinogenesis

Al-Ayyoubi S,Gali-Muhtasib H

MA1-154 was used in western blot to elucidate how treatment with gallotannin prevents cancer

Thu Mar 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: Cyclin A; Cyclin A2 (Cyclin A); Cyclin-A; Cyclin-A2

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Gene Aliases: AA408589; BOS_6308; Ccn-1; CCN1; CCNA; CCNA2; Cyca; CycA2

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UniProt ID: (Bovine) P30274, (Human) P20248, (Mouse) P51943

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Entrez Gene ID: (Bovine) 281667, (Human) 890, (Rat) 114494, (Mouse) 12428

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Function(s)

protein kinase binding protein binding molecular_function

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cyclin A2 Monoclonal Antibody (E23.1)

Cyclin A2 Antibody (MA1-154) in ICC/IF

Product Details

MA1-154

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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