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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Cyclin D1 Antibodies

          Invitrogen

          Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4)

          Advanced Verification
          18 Published Figures
          294 References
          View all (113) Cyclin D1 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4)

          • Antibody Testing Data (12)
          • Published Figures (18)
          • Advanced Verification (2)
          Cyclin D1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Cyclin D1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 32

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          Cyclin D1 Antibody (MA5-14512) in ICC/IF

          Immunofluorescence analysis of was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4) (Product # MA5-14512) at 1:200 dilution in 0.1% BSA, incubated a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Cyclin D1 Antibody in Immunocytochemistry (ICC/IF)
          Cyclin D1 Antibody in Immunocytochemistry (ICC/IF)
          Cyclin D1 Antibody in Immunocytochemistry (ICC/IF)
          Cyclin D1 Antibody in Immunocytochemistry (ICC/IF)
          Cyclin D1 Antibody in Immunocytochemistry (ICC/IF)
          Cyclin D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cyclin D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cyclin D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cyclin D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cyclin D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Immunohistochemistry (IHC)
          Cyclin D1 Antibody in Immunohistochemistry (IHC)
          Cyclin D1 Antibody in Immunohistochemistry (IHC)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody in Western Blot (WB)
          Cyclin D1 Antibody
          Cyclin D1 Antibody

          Product Details

          MA5-14512

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 45 publications 45 publications

          Immunohistochemistry (IHC)

          -
          View 217 publications 217 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          View 20 publications 20 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          1:50-1:250
          View 7 publications 7 publications

          Flow Cytometry (Flow)

          1:30
          -

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Baboon, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          proprietary

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          SP4

          Immunogen

          A synthetic peptide from C-terminus of human cyclin D1
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.045 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.2, with 1% BSA

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10985779

          Product Specific Information

          MA5-14512 targets Cyclin D1/Bcl-1 in IHC (P), ICC/IF and WB applications and shows reactivity with Human and Mouse samples.

          The MA5-14512 immunogen is a synthetic peptide from C-terminus of human cyclin D1.

          Target Information

          Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; CCND1 protein; cDNA; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD-1; PRAD1 oncogene

          View more View less

          Gene Aliases: AI327039; bcl-1; BCL1; CCND1; cD1; CycD1; Cyl-1; D11S287E; PRAD1; U21B31

          View more View less

          UniProt ID: (Human) P24385, (Rat) P39948, (Mouse) P25322

          View more View less

          Entrez Gene ID: (Human) 595, (Rat) 58919, (Mouse) 12443

          View more View less

          Function(s)
          transcription corepressor activity protein kinase activity protein binding transcription factor binding cyclin-dependent protein serine/threonine kinase regulator activity enzyme binding protein kinase binding protein complex binding histone deacetylase binding proline-rich region binding kinase activity
          Process(es)
          G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter re-entry into mitotic cell cycle liver development positive regulation of protein phosphorylation transcription, DNA-templated protein phosphorylation cellular response to DNA damage stimulus lactation positive regulation of cell proliferation response to organic substance response to iron ion response to X-ray response to organonitrogen compound positive regulation of G2/M transition of mitotic cell cycle response to organic cyclic compound Wnt signaling pathway negative regulation of Wnt signaling pathway negative regulation of epithelial cell differentiation endoplasmic reticulum unfolded protein response organ regeneration mitotic G1 DNA damage checkpoint response to magnesium ion response to estradiol response to vitamin E Leydig cell differentiation mammary gland epithelial cell proliferation positive regulation of mammary gland epithelial cell proliferation response to drug response to estrogen fat cell differentiation response to ethanol positive regulation of cyclin-dependent protein serine/threonine kinase activity regulation of protein kinase activity response to steroid hormone cell division response to glucocorticoid response to corticosterone response to calcium ion regulation of cell cycle canonical Wnt signaling pathway mammary gland alveolus development response to UV-A negative regulation of cell cycle arrest cellular response to organic substance regulation of G1/S transition of mitotic cell cycle response to leptin positive regulation of cell cycle liver regeneration regulation of transcription, DNA-templated cell cycle
          It has to be done as per old AB suggested Products section.
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