Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Cyclin D3 Recombinant Rabbit Monoclonal Antibody (4C5C8)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
Cyclin D3 Antibody (MA5-35429) in WB
Western blot analysis of Cyclin D3 in extracts of various cell lines. Samples were incubated with Cyclin D3 Monoclonal antibody (Product # MA5-35429) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
MA5-35429
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4C5C8
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 202-292 of human Cyclin D3 (P30281)
Conjugate
Form
Liquid
Concentration
0.19 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849330
Product Specific Information
Immunogen sequence: MIATGSIGAA VQGLGACSMS GDELTELLAG ITGTEVDCLR ACQEQIEAAL RESLREASQT SSSPAPKAPR GSSSQGPSQT STPTDVTAIH L
Target Information
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. Diseases associated with CCND3 include Retinoblastoma and Plasma Cell Leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CCND 3; CGD3; CyclinD3; D3 type cyclin; D3-type cyclin; G1/S-specific cyclin-D3; OTTHUMP00000223415; RP5-973N23.3
Gene Aliases: 9230106B05Rik; AA682053; AL024085; AW146355; C78795; CCND3; Cyl-3
UniProt ID: (Human) P30281, (Mouse) P30282, (Rat) P48961
Entrez Gene ID: (Human) 896, (Mouse) 12445, (Rat) 25193
positive regulation of protein phosphorylation
protein phosphorylation
cell cycle
signal transduction
positive regulation of cell proliferation
hyaluronan biosynthetic process
T cell proliferation
response to peptide hormone
positive regulation of cyclin-dependent protein serine/threonine kinase activity
cell division
regulation of cell cycle
G1/S transition of mitotic cell cycle
response to glucose
response to organic substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support