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Cyclin L2 Antibody (600-401-878) in WB
Western blot using Rocklands Affinity Purified anti-Cyclin L2a antibody shows detection of a band ~38 kDa corresponding to a GST-truncated human Cyclin L2a fusion protein (arrowhead). Approximately 5 ul of an induced E.coli cell lysate expressing recombinant Cyclin L2 was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] ... View More
Product Details
600-401-878
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 309-384 of Human Cyclin L2a protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human Cyclin L2a and shows negligible reactivity to Cyclin L2b or Cyclin L1 isoforms. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80% homology with the protein from these sources. Cross reactivity with Cyclin L2a homologues from other sources has not been determined.
Target Information
CCNL2 belongs to the cyclin family, Cyclin L subfamily. CCNL2 is a transcriptional regulator which participates in regulating the pre-mRNA splicing process. CCNL2 also modulates the expression of critical apoptotic factor, leading to cell apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cyclin Ania-6b; cyclin M; cyclin S; Cyclin-L2; paneth cell enhanced expression; Paneth cell-enhanced expression protein; PCEE
Gene Aliases: 1700010A01Rik; 1810019L15Rik; 2010319M22Rik; AA409936; ANIA-6B; Ania6b; AW261738; CCNL2; CCNM; CCNS; HCLA-150; HCLA-ISO; HLA-ISO; MNCb-5160; PCEE; SB138
UniProt ID: (Human) Q96S94, (Mouse) Q9JJA7
Entrez Gene ID: (Human) 81669, (Mouse) 56036
transcription, DNA-templated
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of phosphorylation of RNA polymerase II C-terminal domain
regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of transcription, DNA-templated
biological_process
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