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Cyclin T1 Antibody (PA5-77892) in ICC/IF
Cyclin T1 Polyclonal Antibody detects Cyclin T1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin T1 protein stained by Cyclin T1 Polyclonal Antibody (Product # PA5-77892) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Product Details
PA5-77892
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cyclin T1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735698
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Mouse (86%), Rat (87%), Chimpanzee (100%), Bovine (84%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK9-associated C-type protein; cyclin C-related protein; cyclin T1b; Cyclin-T1; CycT1; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
Gene Aliases: CCNT; CCNT1; CYCT1; HIVE1
UniProt ID: (Human) O60563
Entrez Gene ID: (Human) 904, (Rat) 315291
DNA binding
chromatin binding
protein serine/threonine kinase activity
protein binding
transcription factor binding
cyclin-dependent protein serine/threonine kinase regulator activity
transcription regulatory region DNA binding
RNA polymerase binding
7SK snRNA binding
molecular_function
snRNA binding
protein kinase binding
regulation of cyclin-dependent protein serine/threonine kinase activity
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
biological_process
response to drug
negative regulation of mRNA polyadenylation
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
cell cycle
viral process
snRNA transcription from RNA polymerase II promoter
positive regulation by host of viral transcription
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
cell division
positive regulation of phosphorylation of RNA polymerase II C-terminal domain
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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