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Cyclin T2 Antibody (100-401-931) in WB
Western blot using Rocklands Anti-Cyclin T2 (Rabbit) is shown to detect two major bands (arrowheads) corresponding to human Cyclin T2a (~74kDa) and T2b (~81kDa) as indicated. Approximately 33 µg of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% BLOTTO (p/n B501-0500) in PBS, the membrane was probed for overnight at 4° C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG... View More
Product Details
100-401-931
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-584 of human Cyclin T2a protein.
Conjugate
Form
Liquid
Concentration
85 mg/mL
Storage buffer
whole serum
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is directed against human Cyclin T2 proteins. Antiserum will specifically react with proteins 74 and 81 kDa in size corresponding to isoforms of Cyclin T2 protein by western blot. A protein:protein BLAST analysis was used to suggest cross reactivity with Cyclin T2a and T2b from dog (96%), bovine (93%), mouse (88%) and rat (85%) sources based on high homology with the full length protein. Reactivity against homologues from other sources is not known.
Target Information
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. A pseudogene of this gene is found on chromosome 1. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cyclin T2a; cyclin T2b; Cyclin-T2; CycT2; SDS-stable vimentin-bound DNA fragment HEF42VIM22; subunit of positive elongation transcription factor b
Gene Aliases: CCNT2; CYCT2
UniProt ID: (Human) O60583
Entrez Gene ID: (Human) 905
regulation of cyclin-dependent protein serine/threonine kinase activity
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
protein phosphorylation
cell cycle
early viral transcription
late viral transcription
snRNA transcription from RNA polymerase II promoter
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of transcription from RNA polymerase II promoter
cell division
positive regulation of phosphorylation of RNA polymerase II C-terminal domain
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