Invitrogen

Cyclophilin A Polyclonal Antibody

Cyclophilin A Antibody in Immunocytochemistry (ICC/IF)

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Cyclophilin A Antibody (PA1-025) in ICC/IF

Immunofluorescence analysis of Cyclophilin A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclophilin A Rabbit Polyclonal Antibody (Product # PA1-025) at 1:250 dilution in 0.1% BSA and incubated for ... View More

Cyclophilin A Antibody in Western Blot (WB)

Cyclophilin A Antibody in Immunomicroscopy (IM)

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Product Details

PA1-025

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:3000

View 14 publications 14 publications

Immunocytochemistry (ICC/IF)

1:250

View 1 publication 1 publication

in situ PLA (PLA)

View 1 publication 1 publication

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified recombinant human CyPA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2169124

Product Specific Information

PA1-025 detects recombinant human cyclophilin A (CyPA), but does not detect endogenous levels of CyPA. PA1-025 also detects the CyPA protein from CHO cells.

PA1-025 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a prominent ~18 kDa protein representing recombinant human CyPA.

The PA1-025 immunogen is recombinant human CyPA expressed in E. coli.

Target Information

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NCF1-dependent production of ROS protects against lupus by regulating plasmacytoid dendritic cell development and functions.

JCI insight

Luo H,Urbonaviciute V,Saei AA,Lyu H,Gaetani M,Végvári Á,Li Y,Zubarev RA,Holmdahl R

PA1-025 was used in Western Blotting to identify the key immune cell type(s) and cellular mechanisms driving lupus pathogenesis under the condition of NCF1-dependent ROS deficiency.

Mon Apr 10 00:00:00 UTC 2023

p53 restoration in small cell lung cancer identifies a latent cyclophilin-dependent necrosis mechanism.

Nature communications

Acosta J,Li Q,Freeburg NF,Murali N,Indeglia A,Grothusen GP,Cicchini M,Mai H,Gladstein AC,Adler KM,Doerig KR,Li J,Ruiz-Torres M,Manning KL,Stanger BZ,Busino L,Murphy M,Wan L,Feldser DM

PA1-025 was used in Proximity Ligation Assay (PLA) to demonstrate that intertumoral heterogeneity in SCLC influences the biological response to p53 restoration, describes a cyclophilin-dependent mechanism of p53-regulated cell death, and uncovers putative mechanisms for the treatment of this most-recalcitrant tumor type.

Fri Jul 21 00:00:00 UTC 2023

Neutrophil-derived reactive oxygen species promote tumor colonization.

Communications biology

Zhong J,Li Q,Luo H,Holmdahl R

PA1-025 was used in Western Blot to demonstrate that tumor colonization is counteracted by ROS-activated and IL-1β-secreting tissue neutrophils.

Tue Jul 13 00:00:00 UTC 2021

KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection.

PLoS pathogens

Dharan A,Talley S,Tripathi A,Mamede JI,Majetschak M,Hope TJ,Campbell EM

PA1-025 was used in western blot to assess mediation of the nuclear import of HIV-1 during infection by cooperation between Nup358 and KIF5B

Wed Jun 01 00:00:00 UTC 2016

Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74.

Journal of virology

Saito A,Ferhadian D,Sowd GA,Serrao E,Shi J,Halambage UD,Teng S,Soto J,Siddiqui MA,Engelman AN,Aiken C,Yamashita M

PA1-025 was used in western blot to study multimodal inhibition of HIV-1 by PF74 and the roles of capsid-interacting host factors

Wed Jun 15 00:00:00 UTC 2016

In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects.

PLoS pathogens

Henning MS,Dubose BN,Burse MJ,Aiken C,Yamashita M

PA1-025 was used in western blot to investigate HIV-1 capsid protein modulation

Wed Jan 01 00:00:00 UTC 2014

Multiple sites in the N-terminal half of simian immunodeficiency virus capsid protein contribute to evasion from rhesus monkey TRIM5α-mediated restriction.

Retrovirology

Kono K,Song H,Yokoyama M,Sato H,Shioda T,Nakayama EE

PA1-025 was used in western blot to investigate the mechanism for the evasion of Rh TRIM5alpha restriction by simian immunodeficiency viru core protein

Wed Sep 08 00:00:00 UTC 2010

Proteomic profiling identifies cyclooxygenase-2-independent global proteomic changes by celecoxib in colorectal cancer cells.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology

Lou J,Fatima N,Xiao Z,Stauffer S,Smythers G,Greenwald P,Ali IU

PA1-025 was used in western blot to find new celecoxib-mediated markers in the COX-2-deficient colorectal cancer cell line

Fri Sep 01 00:00:00 UTC 2006

Cyclophilin interactions with incoming human immunodeficiency virus type 1 capsids with opposing effects on infectivity in human cells.

Journal of virology

Hatziioannou T,Perez-Caballero D,Cowan S,Bieniasz PD

PA1-025 was used in western blot to investigate the role of CypA-CA interaction in regulating HIV-1 replication and infectivity in target human cells.

Sat Jan 01 00:00:00 UTC 2005

Cell surface expression of CD147/EMMPRIN is regulated by cyclophilin 60.

The Journal of biological chemistry

Pushkarsky T,Yurchenko V,Vanpouille C,Brichacek B,Vaisman I,Hatakeyama S,Nakayama KI,Sherry B,Bukrinsky MI

PA1-025 was used in western blot to demonstrate the role of Cyp60 in the CD147 translocation to the cell surface.

Fri Jul 29 00:00:00 UTC 2005

The characterization and hormonal regulation of kidney androgen-regulated protein (Kap)-luciferase transgenic mice.

Toxicological sciences : an official journal of the Society of Toxicology

Malstrom SE,Tornavaca O,Meseguer A,Purchio AF,West DB

PA1-025 was used in western blot to study the expression of kidney androgen regulated protein (Kap) in Kap-luc transgenic mice

Tue Jun 01 00:00:00 UTC 2004

Redistribution of cyclophilin A to viral factories during vaccinia virus infection and its incorporation into mature particles.

Journal of virology

Castro AP,Carvalho TM,Moussatché N,Damaso CR

PA1-025 was used in western blot and immunocytochemistry to investigate the expression pattern and intracellular distribution of CypA during vaccinia virus infection.

Fri Aug 01 00:00:00 UTC 2003

Redistribution of cyclophilin A to viral factories during vaccinia virus infection and its incorporation into mature particles.

Journal of virology

Castro AP,Carvalho TM,Moussatché N,Damaso CR

PA1-025 was used in western blot and immunocytochemistry to investigate the expression pattern and intracellular distribution of CypA during vaccinia virus infection.

Fri Aug 01 00:00:00 UTC 2003

Redistribution of cyclophilin A to viral factories during vaccinia virus infection and its incorporation into mature particles.

Journal of virology

Castro AP,Carvalho TM,Moussatché N,Damaso CR

PA1-025 was used in western blot and immunocytochemistry to investigate the expression pattern and intracellular distribution of CypA during vaccinia virus infection.

Fri Aug 01 00:00:00 UTC 2003

Cyclophilin A peptidyl-prolyl isomerase activity promotes ZPR1 nuclear export.

Molecular and cellular biology

Ansari H,Greco G,Luban J

PA1-025 was used in western blot to investigate the role for Cpr1p in Zpr1p nuclear export and its functional PPIase activity.

Tue Oct 01 00:00:00 UTC 2002

Native recombinant cyclophilins A, B, and C degrade DNA independently of peptidylprolyl cis-trans-isomerase activity. Potential roles of cyclophilins in apoptosis.

The Journal of biological chemistry

Montague JW,Hughes FM,Cidlowski JA

PA1-025 was used in western blot to investigate the DNA degradation by three cyclophilins isoforms

Fri Mar 07 00:00:00 UTC 1997

A calcium-dependent nuclease from apoptotic rat thymocytes is homologous with cyclophilin. Recombinant cyclophilins A, B, and C have nuclease activity.

The Journal of biological chemistry

Montague JW,Gaido ML,Frye C,Cidlowski JA

PA1-025 was used in western blot to investigate the functional properties of NUC18 compared to cyclophilin

Fri Jul 22 00:00:00 UTC 1994

Bioinformatics

Protein Aliases: cyclophilin; Cyclophilin A; Cyclosporin A-binding protein; CyP A; EC 5.2.1.8; epididymis secretory sperm binding protein Li 69p; HGNC:9253; MGC117158; MGC12404; MGC23397; Peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIase; PPIase A; rotamase; Rotamase A; SP18; T cell cyclophilin

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Gene Aliases: 2700098C05; Cphn; CyP-18; CYPA; CYPH; HEL-S-69p; I79_011869; PPIA

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UniProt ID: (Human) P62937, (Mouse) P17742, (Chinese hamster) P14851

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Entrez Gene ID: (Human) 5478, (Mouse) 268373, (Chinese hamster) 100769490

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Function(s)

peptidyl-prolyl cis-trans isomerase activity protein binding peptide binding poly(A) RNA binding virion binding unfolded protein binding cyclosporin A binding isomerase activity

Process(es)

protein peptidyl-prolyl isomerization protein folding neuron differentiation lipid particle organization regulation of viral genome replication positive regulation of viral genome replication positive regulation of protein secretion RNA-dependent DNA biosynthetic process viral life cycle uncoating of virus fusion of virus membrane with host plasma membrane virion assembly viral release from host cell entry into host cell leukocyte migration establishment of integrated proviral latency

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cyclophilin A Polyclonal Antibody

Cyclophilin A Antibody (PA1-025) in ICC/IF

Product Details

PA1-025

Western Blot (WB)

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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