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Invitrogen
Cypridina luciferase Polyclonal Antibody
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Cypridina luciferase Antibody (PA1-182) in WB
Western blot analysis of secreted Cypridina luciferase was performed using 293T cells stably expressing Gaussia or Cypridina luciferase. Cells were grown overnight in serum free (B27 supplemented) DMEM with or without 10uM Brefeldin A. Equal amounts (75 µg) of approximately 10x concentrated cell culture medium from treated or untreated cells, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616... View More
Product Details
PA1-182
Product Specifications
Species Reactivity
Luciferase
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified recombinant Cypridina luciferase expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539913
Product Specific Information
Western blot analysis of PA1-182 detects a ~62 kDa protein in the supernatant of Cypridina luciferase-secreting cells.
PA1-182 can be used for activity-independent verification of Cypridina luciferase expression.
Target Information
The Cypridina luciferase is a 62kDa protein from the marine ostracod, Cypridina noctiluca. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. Cypridina luciferase can be measured individually or in multiplex assays, such as the Cypridina-Firefly Luciferase Dual Assay Kit (Product # 16183). The Cypridina luciferase has been codon optimized for enhanced performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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