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Invitrogen
Cytochrome C Polyclonal Antibody
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Product Details
PA1-9586
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified bovine cytochrome c protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2230390
Product Specific Information
PA1-9586 detects Cytochrome C in human, mouse, bovine and rat samples.
PA1-9586 has been successfully used in Western blot and ELISA procedures. By Western blot, PA1-9586 detects a 12 kDa protein representing Cytochrome C. Do not use dry milk in blocking or dilution buffer in your assay. Use BSA (3-5% in blocking and 1-3% in dilution buffers) in your assay.
PA1-9586 immunogen is a purified bovine cytochrome c protein
Store at 4ºC for 1 week, and -20ºC for up to one year.
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CRF; Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1
Gene Aliases: BOS_4320; CYC; CYCS; CYCSA; cytc; HCS; THC4
UniProt ID: (Bovine) P62894, (Human) P99999, (Mouse) P62897, (Rat) P62898
Entrez Gene ID: (Bovine) 510767, (Human) 54205, (Mouse) 13063, (Rat) 25309
apoptotic process
electron transport chain
response to reactive oxygen species
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
protein dephosphorylation
mitochondrion organization
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cellular respiration
intrinsic apoptotic signaling pathway
apoptotic DNA fragmentation
hydrogen peroxide metabolic process
ATP synthesis coupled electron transport
response to ischemia
response to oxidative stress
negative regulation of hydrogen peroxide biosynthetic process
glial cell apoptotic process
positive regulation of cellular respiration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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