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Invitrogen
Cytochrome C Chimeric Recombinant Rabbit Monoclonal Antibody (3.7C4)
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FIGURE: 1 / 1
Cytochrome C Chimeric Antibody (MA5-48254) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Cytochrome C in NIH3T3 cells using Cytochrome C Chimeric Monoclonal Antibody (Product # MA5-48254). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with Cytochrome C Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). P... View More
Product Details
MA5-48254
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3.7C4
Immunogen
This antibody was raised by immunising OVA-primed mice with cytochrome c-OVA.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074696
Product Specific Information
Specificity: This antibody recognises mouse cytochrome c (CYT) which is a part of the respiratory chain responsible for the catalysis of the reduction of oxygen to water.
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CRF; Cytochrome c, somatic; hc1
Gene Aliases: Cycs
UniProt ID: (Mouse) P62897
Entrez Gene ID: (Mouse) 13063
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
apoptotic DNA fragmentation
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
hydrogen peroxide metabolic process
ATP synthesis coupled electron transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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