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Invitrogen
Cytoglobin Polyclonal Antibody
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Cytoglobin Antibody (PA5-79122) in IHC (P)
Immunohistochemistry analysis of Cytoglobin on paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Cytoglobin polyclonal antibody (Product# PA5-79122) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minu... View More
Product Details
PA5-79122
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Cytoglobin recombinant protein (Position: M1-P190).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746238
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat small intestine tissue, mouse small intestine tissue. IHC: mouse intestine tissue, mouse cardiac muscle tissue, rat intestine tissue, human intestinal cancer tissue, human lung cancer tissue.
Target Information
Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytoglobin; HGb; Histoglobin; Nitric oxygen dioxygenase; Nitric oxygen dioxygenase CYGB; Nitrite reductase CYGB; NOD; Pseudoperoxidase CYGB; stellate cell activation associated protein; Stellate cell activation-associated protein; Superoxide dismutase CYGB
Gene Aliases: 3110001K20Rik; CYGB; HGB; Staap; STAP
UniProt ID: (Human) Q8WWM9, (Mouse) Q9CX80, (Rat) Q921A4
Entrez Gene ID: (Human) 114757, (Mouse) 114886, (Rat) 170520
transport
response to oxidative stress
negative regulation of fibroblast migration
oxygen transport
fatty acid oxidation
negative regulation of collagen biosynthetic process
oxidation-reduction process
negative regulation of hepatic stellate cell activation
response to hypoxia
regulation of nitric-oxide synthase activity
cellular oxidant detoxification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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