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Invitrogen
Cytokeratin 10/13 Monoclonal Antibody (EBS-IF-003)
Product Details
MA5-33317
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Cat,
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
EBS-IF-003
Immunogen
Cytoskeleton preparation from HeLa cells
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
purified
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
AB_2815455
Product Specific Information
EBS-IF-003 reacts with 53kDa (CK13) and 56.6kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and noncornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis.
Target Information
Cytokeratins(CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. Skin) and non-keratinizing cells (ie. Mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different CKs in the molecular range of 40-70 k Da and isoelectric points of 5-805 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CK-10; CK-13; cytokeratin 10; cytokeratin 13; Cytokeratin-10; Cytokeratin-13; epithelial keratin 10; Epithelial keratin-10; K10; K13; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13; keratin 13, type I; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 13; Keratin-10; Keratin-13
Gene Aliases: BCIE; BIE; CK-10; CK10; CK13; EHK; K10; K13; KER10; KPP; KRT10; KRT13; WSN2
UniProt ID: (Human) P13645, (Dog) Q6EIZ0, (Human) P13646
Entrez Gene ID: (Human) 3858, (Dog) 491006, (Human) 3860, (Dog) 490983
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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