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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Cytokeratin 12 Antibodies

          Invitrogen

          Cytokeratin 12 Recombinant Rabbit Monoclonal Antibody (JE55-35)

          View all (12) Cytokeratin 12 antibodies
          Datasheet
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          Datasheet
          Protocols
          Questions & Answers

          Cite Cytokeratin 12 Recombinant Rabbit Monoclonal Antibody (JE55-35)

          • Antibody Testing Data (2)
          Cytokeratin 12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Cytokeratin 12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          FIGURE: 1 / 2

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          Cytokeratin 12 Antibody (MA5-42505) in IHC (P)

          Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat eyeball tissue using Cytokeratin 12 Recombinant Monoclonal Antibody (Product # MA5-42505). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the Cytokeratin 1... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Cytokeratin 12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cytokeratin 12 Antibody in Western Blot (WB)

          Product Details

          MA5-42505

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          JE55-35

          Immunogen

          Recombinant protein within C-terminal Human Keratin 12/K12
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          TBS, pH 7.4, with 40% glycerol, 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2911646

          Product Specific Information

          Positive Control: Mouse eyeball tissue lysates, rat eyeball tissue.

          Subcellular Location: Intermediate filament, cytosol, extracellular exosome.

          Target Information

          KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: CK-12; Cytokeratin-12; K12; keratin 12 (Meesmann corneal dystrophy); keratin 12, type I; keratin complex 1, acidic, gene 12; keratin K12; Keratin, type I cytoskeletal 12; Keratin, type I cytoskeletal 12 (Cytokeratin 12); Keratin-12; krt12 {ECO:0000250|UniProtKB:Q99456}; type I keratin KA12

          View more View less

          Gene Aliases: AI835216; K12; Ka12; Krt-12; Krt1-12; KRT12

          View more View less

          UniProt ID: (Human) Q99456

          View more View less

          Entrez Gene ID: (Human) 3859, (Mouse) 268482, (Rat) 360625

          View more View less

          Function(s)
          structural molecule activity molecular_function
          Process(es)
          epithelium development cornea development in camera-type eye visual perception biological_process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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