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          • Primary Antibodies ›
          • Cytokeratin 18 Antibodies

          Invitrogen

          Cytokeratin 18 Monoclonal Antibody (DC-10)

          Advanced Verification
          33 References
          View all (157) Cytokeratin 18 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite Cytokeratin 18 Monoclonal Antibody (DC-10)

          • Antibody Testing Data (6)
          • Advanced Verification (2)
          Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
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          Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 8

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          Cytokeratin 18 Antibody (MA1-19041) in ICC/IF

          Immunofluorescence analysis of Cytokeratin 18 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 18 Monoclonal Antibody (DC-10) (Product # MA1-19041) at a concentration of 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and th... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
          Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF)
          Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cytokeratin 18 Antibody in Western Blot (WB)
          Cytokeratin 18 Antibody in Western Blot (WB)
          Cytokeratin 18 Antibody in Flow Cytometry (Flow)
          Cytokeratin 18 Antibody
          Cytokeratin 18 Antibody

          Product Details

          MA1-19041

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 14 publications 14 publications

          Immunohistochemistry (IHC)

          -
          View 11 publications 11 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          10 µg/mL
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          1-4 µg/mL
          View 1 publication 1 publication

          ELISA (ELISA)

          Assay-dependent
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Non-human primate, Rhesus monkey

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          DC-10

          Immunogen

          Human breast carcinoma cell line PMC-42.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • FITC
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          15mM sodium azide

          Storage conditions

          4°C, do not freeze

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1075168

          Product Specific Information

          This antibody reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.

          This antibody will not cross-react with porcine, mouse, rat, hamster, bovine, canine or sheep.

          Target Information

          Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin-18; Cytokeratin18; K 18; K18; keratin 18, type I; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; type I cytoskeletal 18

          View more View less

          Gene Aliases: CK-18; CYK18; K18; KRT18; PIG46

          View more View less

          UniProt ID: (Human) P05783

          View more View less

          Entrez Gene ID: (Human) 3875

          View more View less

          Function(s)
          structural molecule activity protein binding poly(A) RNA binding scaffold protein binding cadherin binding involved in cell-cell adhesion
          Process(es)
          cell cycle anatomical structure morphogenesis viral process tumor necrosis factor-mediated signaling pathway Golgi to plasma membrane CFTR protein transport negative regulation of apoptotic process intermediate filament cytoskeleton organization extrinsic apoptotic signaling pathway hepatocyte apoptotic process cell-cell adhesion
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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