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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Cytokeratin 19 Antibodies

          Invitrogen

          Cytokeratin 19 Monoclonal Antibody (A53-B/A2.26 (Ks19.1))

          18 Published Figures
          52 References
          View all (166) Cytokeratin 19 antibodies

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          Datasheet
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          Datasheet
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          Cite Cytokeratin 19 Monoclonal Antibody (A53-B/A2.26 (Ks19.1))

          • Antibody Testing Data (4)
          • Published Figures (18)
          Cytokeratin 19 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Cytokeratin 19 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 22

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          Cytokeratin 19 Antibody (MA5-12663) in ICC/IF

          Immunofluorescent analysis of Cytokeratin 19 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 (A53-B/A2.26 (Ks19.1)) Mouse Monoclonal Antibody (Product # MA5-12663) at 2 µg/mL in 0.1% BSA... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Cytokeratin 19 Antibody in Immunocytochemistry (ICC/IF)
          Cytokeratin 19 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cytokeratin 19 Antibody in Western Blot (WB)
          Cytokeratin 19 Antibody in Western Blot (WB)
          Cytokeratin 19 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Cytokeratin 19 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Cytokeratin 19 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))
          Cytokeratin 19 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cytokeratin 19 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 19 Antibody in Immunohistochemistry (IHC)

          Product Details

          MA5-12663

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          2 µg/mL
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          -
          View 23 publications 23 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:200
          View 9 publications 9 publications

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 13 publications 13 publications

          Dot blot (DB)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Cat, Human, Mouse, Rhesus monkey, Sheep

          Host/Isotype

          Mouse / IgG2a, lambda

          Class

          Monoclonal

          Type

          Antibody

          Clone

          A53-B/A2.26 (Ks19.1)

          Immunogen

          Human breast cancer MCF-7 cells
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10984317

          Product Specific Information

          MA5-12663 targets Cytokeratin 19 in IF, IHC (P), and WB applications and shows reactivity with Human samples.

          The MA5-12663 immunogen is human breast cancer MCF-7 cells.

          Target Information

          Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 40-kDa keratin intermediate filament; C K1CS; CK 19; CK-19; cytokeratin 19; Cytokeratin-19; K19; keratin 19, type I; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin-19; MGC15366

          View more View less

          Gene Aliases: CK19; K19; K1CS; KRT19

          View more View less

          UniProt ID: (Human) P08727

          View more View less

          Entrez Gene ID: (Human) 3880

          View more View less

          Function(s)
          structural constituent of cytoskeleton protein binding structural constituent of muscle protein complex binding
          Process(es)
          Notch signaling pathway viral process response to estrogen sarcomere organization cell differentiation involved in embryonic placenta development
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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