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Product Details
65031
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
C22
Immunogen
Human Keratin K8, Purified From Sds Page Gel
Conjugate
Form
Liquid
Concentration
5 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.5% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
C 22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. Polypeptide reacting: MW 52 500, MW 58 000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells.
Target Information
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 58 kDa cytokeratin; CK-5; CK-8; cytokeratin 5; cytokeratin 8; Cytokeratin endo A; Cytokeratin-5; Cytokeratin-8; Cytokeratin-A; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; K8; KERA; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 8, type II; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 5; keratin, type II cytoskeletal 60 kDa, component III; Keratin, type II cytoskeletal 8; Keratin-5; Keratin-8; krt5; tuftelin interacting protein 8; Type-II keratin Kb5; Type-II keratin Kb8
Gene Aliases: 3300001P10Rik; AA960620; AL022697; AU019895; AW146334; BOS_5040; BOS_5041; CARD2; CK-8; CK5; CK8; CYK8; DDD; DDD1; EBS2; EndoA; K2C8; K5; K8; KO; Krt-2.8; Krt2-5; KRT2-8; KRT5; KRT5A; KRT8; Tfip8
UniProt ID: (Bovine) Q5XQN5, (Human) P13647, (Bovine) P05786, (Human) P05787, (Mouse) Q922U2, (Mouse) P11679
Entrez Gene ID: (Bovine) 281268, (Human) 3852, (Bovine) 281269, (Human) 3856, (Mouse) 110308, (Mouse) 16691
epithelial cell differentiation
negative regulation of epithelial cell proliferation
cell morphogenesis involved in differentiation
multicellular organism development
tumor necrosis factor-mediated signaling pathway
sarcomere organization
response to hydrostatic pressure
response to other organism
cell differentiation involved in embryonic placenta development
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
viral process
cytoskeleton organization
epidermis development
hemidesmosome assembly
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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