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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Cytokeratin 5 Antibodies

          Invitrogen

          Cytokeratin 5 Monoclonal Antibody (XM26)

          10 Published Figures
          47 References
          View all (78) Cytokeratin 5 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite Cytokeratin 5 Monoclonal Antibody (XM26)

          • Antibody Testing Data (8)
          • Published Figures (10)
          Cytokeratin 5 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Cytokeratin 5 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 18

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          Cytokeratin 5 Antibody (MA5-12596) in ICC/IF

          Immunofluorescence analysis of Cytokeratin 5 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 5 (XM26) Mouse Monoclonal Antibody (Product # MA5-12596) at 1:250 dilution in 0.1% BSA and incub... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Cytokeratin 5 Antibody in Immunocytochemistry (ICC/IF)
          Cytokeratin 5 Antibody in Immunocytochemistry (ICC/IF)
          Cytokeratin 5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cytokeratin 5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cytokeratin 5 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Cytokeratin 5 Antibody in Western Blot (WB)
          Cytokeratin 5 Antibody in Western Blot (WB)
          Cytokeratin 5 Antibody in Western Blot (WB)
          Cytokeratin 5 Antibody in Immunocytochemistry (ICC/IF)
          Cytokeratin 5 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))
          Cytokeratin 5 Antibody in Immunocytochemistry (ICC/IF)
          Cytokeratin 5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cytokeratin 5 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 5 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 5 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 5 Antibody in Immunohistochemistry (IHC)
          Cytokeratin 5 Antibody in Western Blot (WB)
          Cytokeratin 5 Antibody in Western Blot (WB)

          Product Details

          MA5-12596

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 29 publications 29 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:100
          View 6 publications 6 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:200-1:250
          View 8 publications 8 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          In vitro Assay (IV)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          XM26

          Immunogen

          Recombinant protein corresponding to C-terminal 103 aa of cytokeratin 5
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          tissue culture supernatant

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11008032

          Product Specific Information

          MA5-12596 targets Cytokeratin 5 in IHC (P) applications and shows reactivity with Human samples.

          The MA5-12596 immunogen is recombinant protein corresponding to C-terminal 103 aa of cytokeratin 5.

          Target Information

          Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 58 kDa cytokeratin; CK-5; Cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; Keratin, type II cytoskeletal 5; Keratin-5; Type-II keratin Kb5

          View more View less

          Gene Aliases: CK5; DDD; DDD1; EBS2; K5; KRT5; KRT5A

          View more View less

          UniProt ID: (Human) P13647

          View more View less

          Entrez Gene ID: (Human) 3852

          View more View less

          Function(s)
          structural constituent of cytoskeleton protein binding scaffold protein binding
          Process(es)
          cytoskeleton organization epidermis development hemidesmosome assembly
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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