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Invitrogen
Cytokeratin 8 Polyclonal Antibody
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Cytokeratin 8 Antibody (PA5-95435) in IHC (P)
Immunohistochemistry (Paraffin) analysis of Cytokeratin 8 in paraffin-embedded section of human thyroid cancer tissue using Cytokeratin 8 Polyclonal Antibody (Product # PA5-95435). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 5 µg/mL d... View More
Product Details
PA5-95435
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807238
Product Specific Information
Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, mouse ovary tissue, mouse liver tissue, MCF-7 whole cell, A549 whole cell, HELA whole cell. IHC: mouse liver tissue, rat intestine tissue, human intestinal cancer tissue, human stomach cancer tissue, human endometrial cancer tissue, human ovarian cancer tissue, human thyroid cancer tissue, human pancreas cancer tissue, human appendix cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CK-8; cytokeratin 8; Cytokeratin endo A; Cytokeratin-8; K8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8
Gene Aliases: AA960620; AL022697; AU019895; CARD2; CK-8; CK8; CYK8; CYKER; EndoA; K2C8; K8; KO; Krt-2.8; Krt2-8; KRT8
UniProt ID: (Human) P05787, (Mouse) P11679, (Rat) Q10758
Entrez Gene ID: (Human) 3856, (Mouse) 16691, (Rat) 25626
cell morphogenesis involved in differentiation
multicellular organism development
tumor necrosis factor-mediated signaling pathway
sarcomere organization
response to hydrostatic pressure
response to other organism
cell differentiation involved in embryonic placenta development
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
viral process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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