Invitrogen

Cytokeratin HMW Monoclonal Antibody (34BetaE12)

Cytokeratin HMW Antibody in Immunocytochemistry (ICC/IF)

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Cytokeratin HMW Antibody (MA5-12135) in ICC/IF

Immunofluorescence analysis of Cytokeratin HMW was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with of Cytokeratin HMW (34BetaE12) Mouse Monoclonal Antibody (Product # MA5-12135) at 1:250 dilution in0.1% BSA and inc... View More

Cytokeratin HMW Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytokeratin HMW Antibody in Western Blot (WB)

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Product Details

MA5-12135

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

Immunohistochemistry (IHC)

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Horse, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

34BetaE12

Immunogen

Solubilized keratin extracted from human stratum corneum

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10983918

Product Specific Information

MA5-12135 targets Cytokeratin HMW and it recognizes keratin 1, 5, 10, and 14 in the Moll catalog (MW 68kDa, 58kDa, 56.5kDa, and 50kDa), respectively. The antibody has been tested in ICC/IF, WB and IHC (P) applications and shows reactivity with Equine, Human, Non-human primate, and Rabbit samples. This antibody does not react with rat tissue in Western blot applications.

In normal cells, Cytokeratin HMW labels squamous, ductal and other complex epithelia. It reacts with benign small-acinir lesions of the prostate and does not react with hepatocytes, pancreatic acinar cells, proximal renal tubes or endometrial glands. In tumor cells, this antibody is reactive with both squamous and ductal neoplasms and variably with those derived from simple epithelium.

The MA5-12135 immunogen is solubilized keratin extracted from human stratum corneum.

Target Information

Keratins are heteropolymeric structural proteins that form the intermediate filament. Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of cytokeratins - the low weight, acidic type I and the high weight, basic type II. Cytokeratins are usually found in pairs - one type I and one type II. KRT1 regulates the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase I (RACK1). In complex with C1QBP it is a high affinity receptor for kininogen-1/HMWK. KRT1 is expressed in the spinous and granular layers of the epidermis with family member KRT10. Mutations affecting the gene result in bullous congenital ichthyosiform erythroderma. KRT5 is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations affecting the gene result in epidermolysis bullosa simplex diseases. KRT10 plays a role in the establishment of the epidermal barrier on plantar skin. It also acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB - thus it plays a role in nasal colonization. Mutations affecting the gene result in epidermolytic hyperkeratosis. KRT14’s nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. Mutations affecting the gene result in epidermolysis bullosa simplex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin.

Journal of veterinary internal medicine

Kupczyk P,Rykala M,Serek P,Pawlak A,Slowikowski B,Holysz M,Chodaczek G,Madej JP,Ziolkowski P,Niedzwiedz A

MA5-12135 was used in Immunohistochemistry to represent a starting point for developing and translating new veterinary medicine-based pharmacotherapies using ECS as a possible target.

Fri Jul 01 00:00:00 UTC 2022

Novel Paired Normal Prostate and Prostate Cancer Model Cell Systems Derived from African American Patients.

Cancer research communications

Jung M,Kowalczyk K,Hankins R,Bandi G,Kallakury B,Carrasquilla MA,Banerjee PP,Grindrod S,Dritschilo A

MA5-12135 was used in Immunocytochemistry-immunofluorescence to examine viability of cells following exposure to the antiandrogen (bicalutamide) and two PARP inhibitors (olaparib and niraparib) and observed decreased viability of tumor-derived cells as compared with viability of normal prostate-derived cells.

Thu Dec 01 00:00:00 UTC 2022

Mechanisms of Endogenous HIV-1 Reactivation by Endocervical Epithelial Cells.

Journal of virology

Gornalusse GG,Valdez R,Fenkart G,Vojtech L,Fleming LM,Pandey U,Hughes SM,Levy CN,Dela Cruz EJ,Calienes FL,Kirby AC,Fialkow MF,Lentz GM,Wagoner J,Jing L,Koelle DM,Polyak SJ,Fredricks DN,McElrath MJ,Wald A,Hladik F

MA5-12135 was used in Immunohistochemistry (PFA fixed) to find that the columnar epithelial cells lining the endocervix, the lower part of the uterus, are particularly effective in reactivating HIV-1 from infected T cells.

Thu Apr 16 00:00:00 UTC 2020

Reevaluation of MAML2 fusion-negative mucoepidermoid carcinoma: a subgroup being actually hyalinizing clear cell carcinoma of the salivary gland with EWSR1 translocation.

Human pathology

Hsieh MS,Wang H,Lee YH,Ko JY,Chang YL

MA512135 was used in immunohistochemistry - paraffin section to use MAML2 staining to distinguish cases of hyalinizing clear cell carcinoma from mucoepidermoid carcinoma

Wed Mar 01 00:00:00 UTC 2017

Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Marian C,Boila A,Soanca D,Malau M,Podeanu DM,Resetkova E,Stolnicu S

MA5-12135 was used in immunohistochemistry to report on two cases of malignant transformation of adenomyoepithelioma of the breast by a monophasic population

Mon Apr 01 00:00:00 UTC 2013

Romanowsky-Giemsa as a counterstain for immunohistochemistry: optimizing a traditional reagent.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Stefanović D,Stefanović M,Nikin Z

MA5-12135 was used in immunohistochemistry to develop a protocol for the counterstaining of immunohistochemical sections using Romanowsky-Giemsa

Thu Aug 01 00:00:00 UTC 2013

Sinonasal teratocarcinosarcoma: a clinical and pathological analysis.

International journal of surgical pathology

Yang S,Sun R,Liang J,Zhou Z,Zhou J,Rui J

MA5-12135 was used in immunohistochemistry to report on two cases of sinonasal teratocarcinosarcoma

Fri Feb 01 00:00:00 UTC 2013

Chemical castration and anti-androgens induce differential gene expression in prostate cancer.

The Journal of pathology

Lehmusvaara S,Erkkilä T,Urbanucci A,Waltering K,Seppälä J,Larjo A,Tuominen VJ,Isola J,Kujala P,Lähdesmäki H,Kaipia A,Tammela TLj,Visakorpi T

MA5-12135 was used in immunohistochemistry to study the differential effects of chemical castration and anti-androgens on gene expression in prostate cancer

Sun Jul 01 00:00:00 UTC 2012

Identification of distinct gene expression profiles between esophageal squamous cell carcinoma and adjacent normal epithelial tissues.

The Tohoku journal of experimental medicine

Tao Y,Chai D,Ma L,Zhang T,Feng Z,Cheng Z,Wu S,Qin Y,Lai M

MA5-12135 was used in immunohistochemistry to use cDNA arrays to study the distinct gene expression profiles of esophageal squamous cell carcinoma cells and the surrounding normal tissue

Sun Apr 01 00:00:00 UTC 2012

A clinicopathological study of the significance of the proportion of choroid morphology in chordoid meningioma.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia

Lin JW,Lu CH,Lin WC,Wu YT,Huang YJ,Shih FY,Ho JT,Chuang MJ

MA5-12135 was used in immunohistochemistry to study chordoid menigioma and the significance of the proportion of chordoid morphology for the risk of recurrence

Fri Jun 01 00:00:00 UTC 2012

Clear cell meningioma with frequent chordoid features and aggressive behavior: a clinicopathologic study of ten cases at a single institution.

Journal of neuro-oncology

Chen HK,Wu YT,Lin YJ,Lin JW

MA5-12135 was used in immunohistochemistry to study the clinicopathology of clear cell meningioma with frequent chordoid features and aggressive behavior

Fri Jul 01 00:00:00 UTC 2011

Routine dual-color immunostaining with a 3-antibody cocktail improves the detection of small cancers in prostate needle biopsies.

Human pathology

Tolonen TT,Kujala PM,Laurila M,Tirkkonen M,Ilvesaro J,Tuominen VJ,Tammela TL,Isola J

MA5-12135 was used in immunohistochemistry to evaluate a dual-color immunostaining protocol to detect small cancers in prostate needle biopsies

Tue Nov 01 00:00:00 UTC 2011

The prognostic impact of O6-methylguanine DNA methyltransferase and epidermal growth factor receptor expressions on primary gliosarcoma: a clinicopathologic and immunohistochemical study of seven cases at a single institution.

Indian journal of pathology & microbiology

Lin JW,Wu YT,Chang IW

MA5-12135 was used in immunohistochemistry to study the prognostic value of MGMT and EGFR expression in primary gliosarcoma

Thu Apr 12 00:00:00 UTC 2012

Chordoid meningioma: a clinicopathologic study of 11 cases at a single institution.

Journal of neuro-oncology

Lin JW,Ho JT,Lin YJ,Wu YT

MA5-12135 was used in immunohistochemistry to study the clinicopathology of 11 cases of chordoid meningioma

Wed Dec 01 00:00:00 UTC 2010

Differences in the structural features of atypical adenomatous hyperplasia and low-grade prostatic adenocarcinoma.

Indian journal of urology : IJU : journal of the Urological Society of India

Midi A,Tecimer T,Bozkurt S,Ozkan N

MA5-12135 was used in immunohistochemistry to investigate the diagnostic histological differences between atypical adenomatous hyperplasia and low-grade prostatic adenocarcinoma

Tue Apr 01 00:00:00 UTC 2008

Chronic inflammation in benign prostate hyperplasia is associated with focal upregulation of cyclooxygenase-2, Bcl-2, and cell proliferation in the glandular epithelium.

The Prostate

Wang W,Bergh A,Damber JE

MA5-12135 was used in immunohistochemistry to study the upregulation of inflammatory pathways in benign prostatic hyperplasia and their possible role in prostate cancer pathogenesis

Wed Sep 15 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: 58 kDa cytokeratin; 67 kDa cytokeratin; CK-1; CK-10; CK-14; CK-5; cytokeratin 1; cytokeratin 10; cytokeratin 14; Cytokeratin-1; Cytokeratin-10; Cytokeratin-14; Cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; Hair alpha protein; K1; K10; K14; K5; keratin 1, type II; keratin 10, type I; keratin 14, type I; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 14; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 5; Keratin-1; Keratin-10; Keratin-14; Keratin-5; Type-II keratin Kb1; Type-II keratin Kb5

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Gene Aliases: BCIE; BIE; CK1; CK10; CK14; CK5; DDD; DDD1; EBS2; EBS3; EBS4; EHK; EHK1; EPPK; K1; K10; K14; K5; KPP; KRT1; KRT10; KRT14; KRT1A; KRT5; KRT5A; KRTA; NEPPK; NFJ

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UniProt ID: (Human) P04264, (Human) P13645, (Human) P02533, (Human) P13647

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Entrez Gene ID: (Human) 3848, (Human) 3858, (Human) 3861, (Human) 3852

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Function(s)

receptor activity structural molecule activity protein binding carbohydrate binding structural constituent of epidermis structural constituent of cytoskeleton keratin filament binding scaffold protein binding

Process(es)

complement activation, lectin pathway retina homeostasis response to oxidative stress fibrinolysis regulation of angiogenesis negative regulation of inflammatory response establishment of skin barrier aging epidermis development response to zinc ion response to ionizing radiation epithelial cell differentiation hemidesmosome assembly hair cycle intermediate filament bundle assembly keratinocyte differentiation cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin HMW Monoclonal Antibody (34BetaE12)

Cytokeratin HMW Antibody (MA5-12135) in ICC/IF

Product Details

MA5-12135

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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