Invitrogen

Cytokeratin Pan Type I Monoclonal Antibody (AE1)

Cytokeratin Pan Type I Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

Cytokeratin Pan Type I Antibody (MA5-13144) in ICC/IF

Immunofluorescence analysis of Cytokeratin Low Molecular Weight was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8/18 Monoclonal Antibody (Product # MA5-13144) at 5µg/mL in 0.1% BSA and incub... View More

Cytokeratin Pan Type I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytokeratin Pan Type I Antibody in Western Blot (WB)

Product Image Gallery

Product Details

MA5-13144

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Species Reactivity

Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE1

Immunogen

Human epidermal keratin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10982255

Product Specific Information

MA5-13144 targets Cytokeratin Low Molecular Weight in WB, IF and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, mouse, Non-human primate, Rabbit, and Rat samples. This antibody is not recommended for NIH-3T3 cells in immunofluorescent applications.

The MA5-13144 immunogen is human epidermal keratin.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fumarate induces redox-dependent senescence by modifying glutathione metabolism.

Nature communications

Zheng L,Cardaci S,Jerby L,MacKenzie ED,Sciacovelli M,Johnson TI,Gaude E,King A,Leach JD,Edrada-Ebel R,Hedley A,Morrice NA,Kalna G,Blyth K,Ruppin E,Frezza C,Gottlieb E

MA5-13144 was used in immunohistochemistry - paraffin section to study how redox-dependent senescence is induced by fumarate to modify glutathione metabolism

Fri Jan 23 00:00:00 UTC 2015

Association of Merkel cell polyomavirus infection with EGFR mutation status in Chinese non-small cell lung cancer patients.

Lung cancer (Amsterdam, Netherlands)

Xu S,Jiang J,Yu X,Sheng D,Zhu T,Jin M

MA5-13144 was used in immunohistochemistry to study Chinese non-small cell lung cancer patients to deterine if there is an association between polyomavirus infection of Merkel cells and EGFR hotspot mutations

Sat Mar 01 00:00:00 UTC 2014

A clinicopathological study of the significance of the proportion of choroid morphology in chordoid meningioma.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia

Lin JW,Lu CH,Lin WC,Wu YT,Huang YJ,Shih FY,Ho JT,Chuang MJ

MA5-13144 was used in immunohistochemistry to study chordoid menigioma and the significance of the proportion of chordoid morphology for the risk of recurrence

Fri Jun 01 00:00:00 UTC 2012

Clear cell meningioma with frequent chordoid features and aggressive behavior: a clinicopathologic study of ten cases at a single institution.

Journal of neuro-oncology

Chen HK,Wu YT,Lin YJ,Lin JW

MA5-13144 was used in immunohistochemistry to study the clinicopathology of clear cell meningioma with frequent chordoid features and aggressive behavior

Fri Jul 01 00:00:00 UTC 2011

Rhabdoid papillary meningioma: a clinicopathologic case series study.

Neuropathology : official journal of the Japanese Society of Neuropathology

Wu YT,Ho JT,Lin YJ,Lin JW

MA5-13144 was used in immunohistochemistry to study the clinicopathology of a series of cases of rhabdoid papillary meningioma

Thu Dec 01 00:00:00 UTC 2011

The prognostic impact of O6-methylguanine DNA methyltransferase and epidermal growth factor receptor expressions on primary gliosarcoma: a clinicopathologic and immunohistochemical study of seven cases at a single institution.

Indian journal of pathology & microbiology

Lin JW,Wu YT,Chang IW

MA5-13144 was used in immunohistochemistry to study the prognostic value of MGMT and EGFR expression in primary gliosarcoma

Thu Apr 12 00:00:00 UTC 2012

Novel immunofluorescence protocol for multimarker assessment of putative disseminating breast cancer stem cells.

Applied immunohistochemistry & molecular morphology : AIMM

Balic M,Rapp N,Stanzer S,Lin H,Strutz J,Szkandera J,Daidone MG,Samonigg H,Cote RJ,Dandachi N

MA5-13144 was used in immunohistochemistry to investigate the efficiency of a new method for the assessment of putative disseminating breast cancer stem cells

Sat Jan 01 00:00:00 UTC 2011

Chordoid meningioma: a clinicopathologic study of 11 cases at a single institution.

Journal of neuro-oncology

Lin JW,Ho JT,Lin YJ,Wu YT

MA5-13144 was used in immunohistochemistry to study the clinicopathology of 11 cases of chordoid meningioma

Wed Dec 01 00:00:00 UTC 2010

Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.

Research in veterinary science

Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS

MA5-13144 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines

Thu Apr 01 00:00:00 UTC 2010

Melanotic Xp11 translocation renal cancer: a case with PSF-TFE3 gene fusion and up-regulation of melanogenetic transcripts.

The American journal of surgical pathology

Chang IW,Huang HY,Sung MT

MA5-13144 was used in immunohistochemistry to report on a case of melanotic Xp11 translocation renal cancer

Tue Dec 01 00:00:00 UTC 2009

Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats.

Laboratory animals

Esendagli G,Yilmaz G,Canpinar H,Gunel-Ozcan A,Guc MO,Guc D

MA5-13144 was used in immunohistochemistry to investigate tumourigenesis in non-mammary tissues in the MNU-induced mammary tumour model

Thu Jan 01 00:00:00 UTC 2009

An immunohistochemical study of feline endometrial adenocarcinoma.

Journal of comparative pathology

Gil da Costa RM,Santos M,Amorim I,Lopes C,Pereira PD,Faustino AM

MA5-13144 was used in immunohistochemistry - paraffin section to characterize feline endometrial adenocarcinomas immunohistochemically

Fri May 01 00:00:00 UTC 2009

Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.

International journal of colorectal disease

Yagci G,Unlu A,Kurt B,Can MF,Kaymakcioglu N,Cetiner S,Tufan T,Sen D

MA5-13144 was used in immunohistochemistry to study micrometastases and skip metastases in carcinoma of the colon and rectum

Thu Feb 01 00:00:00 UTC 2007

Mediastinal intraoperative radioisotope sentinel lymph node mapping in non-small-cell lung cancer.

Nuclear medicine communications

Atinkaya C,Ozlem Küçük N,Koparal H,Aras G,Sak SD,Ozdemir N

MA5-13144 was used in immunohistochemistry to develop a method to map sentinel lymph nodes in non-small cell lung cancer patients

Mon Aug 01 00:00:00 UTC 2005

Possible association between HPV16 E7 protein level and cytokeratin 19.

International journal of cancer

Favia G,Kanduc D,Lo Muzio L,Lucchese A,Serpico R

MA5-13144 was used in western blot to study the association between HPV16 E7 protein levels and cytokeratin 19

Mon Sep 20 00:00:00 UTC 2004

Ectopic thyroid in the gallbladder: report of a case.

Surgery today

Ihtiyar E,Isiksoy S,Algin C,Sahin A,Erkasap S,Yasar B

MA5-13144 was used in immunohistochemistry to describe an unusual case of ectopic thyroid tissue

Fri Feb 27 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: 40-kDa keratin intermediate filament; 56 kDa cytokeratin; Acidic Cytokeratin; Cell proliferation-inducing gene 46 protein; CK-10; CK-14; CK-15; CK-16; CK-18; CK-19; CK-9; cytokeratin 10; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 9; Cytokeratin endo B; Cytokeratin VIB; cytokeratin VII; Cytokeratin-10; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-18; Cytokeratin-19; Cytokeratin-6B; cytokeratin-7; Cytokeratin-9; Endo B; epidermal keratin 10; epidermal keratin VII; epidermolysis bullosa simplex, Dowling-Meara, Koebner; epidermolytic hyperkeratosis; keratosis palmaris et plantaris; focal non-epidermolytic palmoplantar keratoderma; GK-19; HMWCK; intermediate filament protein; K10; K14; K15; K16; K18; K19; K9; keratin 10; keratin 10 (epidermolytic hyperkeratosis); keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13-like; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin 14-like; keratin 15; keratin 15, type I; keratin 16, type I; keratin 18, type I; keratin 18-like; keratin 19, type I; keratin 9 (epidermolytic palmoplantar keratoderma); keratin 9, type I; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 1, acidic, gene 9; Keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 59 kDa; Keratin, type I cytoskeletal 9; keratin, type I, 40-kd; Keratin-10; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-18; Keratin-19; Keratin-9; krt10 {ECO:0000250|UniProtKB:P13645}; krt15 {ECO:0000250|UniProtKB:P19012}; krt18 {ECO:0000250|UniProtKB:P05783}; krt9; pan keratin; pankeratin; Spermatid perinuclear ring manchette protein K9; suprabasal cytokeratin 10; type I alpha-keratin 14; type I alpha-keratin 15; type I cytoskeletal 15; type I cytoskeletal 9; type I keratin KA10; Type I keratin Ka14; Type I keratin Ka15; type I keratin KA16; Type I keratin Ka19

View more View less

Gene Aliases: AI324768; AI528832; AI626930; AI663979; BCIE; BIE; BOS_19066; BOS_19076; BOS_19084; BOS_19102; BOS_5019; CK-18; CK-9; CK10; CK14; CK15; CK16; CK18; CK19; CYK18; D130054E02Rik; EBS3; EBS4; EHK; EndoC; EPPK; FNEPPK; GK-19; K10; K14; K15; K16; K18; K19; K1C1; K1CO; K1CP; K1CS; K9; Ka10; Ka14; Ka15; Ka16; Ka19; Kerd; KPP; Krt-1.10; Krt-1.14; Krt-1.19; Krt1-10; Krt1-14; Krt1-15; Krt1-16; Krt1-18; Krt1-19; Krt1-9; KRT10; KRT14; KRT15; KRT16; KRT16A; KRT18; KRT19; KRT9; KRTB; NEPPK; NFJ; PC1; PIG46