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Invitrogen
Cytokeratin Pan Type II Monoclonal Antibody (AE3), eBioscience™
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Product Details
14-9000-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Human,
Mouse,
Non-human primate,
Rabbit,
Rhesus monkey
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
AE3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1834479
Product Specific Information
Description: The monoclonal antibody AE3 recognizes many members of the basic (Type II) cytokeratin family. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these cytokeratins, and their expression can help identify the origin of a neoplasm.
The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins (also known as cytokeratin 1, 2, 3, 4, 5, 6, 7 and 8) in mouse, human, rat, primate (cynomolgus and rhesus), dog, cat, rabbit and chicken.
Applications Reported: This AE3 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This AE3 antibody has been by tested western blot on SDS-reduced lysates from MCF7 cells at less than or equal to 2 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 57kd keratin; 57kDa keratin; 58 kDa cytokeratin; 60-kDa keratin; 65 kDa cytokeratin; 67 kDa cytokeratin; CK-1; CK-2e; CK-2P; CK-3; CK-4; CK-5; CK-6A; CK-6D; CK-7; CK-72; CK-8; cytokeratin 1; cytokeratin 2; cytokeratin 3; cytokeratin 4; cytokeratin 5; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin 7; cytokeratin 8; Cytokeratin endo A; Cytokeratin otokeratin; Cytokeratin-1; Cytokeratin-2e; Cytokeratin-2P; Cytokeratin-3; Cytokeratin-4; Cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Cytokeratin-7; Cytokeratin-72; Cytokeratin-8; Cytoskeletal 57 kDa keratin; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolytic hyperkeratosis 1; epithelial keratin 1; epithelial keratin 2e; Epithelial keratin-1; Epithelial keratin-2e; Hair alpha protein; K1; K2e; K2P; K3; K3 keratin; K4; K5; K6A; K7; K72; K76; K8; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 1; keratin 1 (epidermolytic hyperkeratosis); keratin 1, type II; keratin 2; keratin 2 epidermis; keratin 2, type II; keratin 2A (epidermal ichthyosis bullosa of Siemens); keratin 2p; keratin 3; keratin 3, type II; keratin 4; keratin 4, type II; keratin 5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 5-like; keratin 6, inner root sheath, 2; keratin 6A; keratin 6A, , type II; keratin 6A, type II; keratin 6A-like; keratin 6B; keratin 7; keratin 7, type II; keratin 72; keratin 72, pseudogene; keratin 72, type II; keratin 75; keratin 76, type II; keratin 8; keratin 8, type II; keratin 86; keratin complex 2, basic, gene 1; keratin complex 2, basic, gene 17; keratin complex 2, basic, gene 35; keratin complex 2, basic, gene 4; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, basic, gene 7; keratin complex 2, basic, gene 8; keratin complex 2, gene 17; keratin complex 2, gene 6a; keratin K3; keratin K6h; keratin protein K6irs; keratin, 55K type II cytoskeletal; keratin, epidermal type II, K6A; keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 2 epidermal; Keratin, type II cytoskeletal 2 oral; keratin, type II cytoskeletal 2 oral-like; Keratin, type II cytoskeletal 3; keratin, type II cytoskeletal 3-like; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; keratin, type II cytoskeletal 5-like; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 7; Keratin, type II cytoskeletal 72; keratin, type II cytoskeletal 72-like; Keratin, type II cytoskeletal 8; Keratin, type II cytoskeletal cochleal; Keratin-1; Keratin-2 epidermis; Keratin-2e; Keratin-3; Keratin-4; Keratin-5; Keratin-6-alpha; Keratin-6A; keratin-6C; Keratin-7; Keratin-72; Keratin-76; Keratin-8; KRT1; krt2; krt5; krt7; krt76; masticatory epithelia keratin 2p; MGC116967; MGC116968; mK6-alpha; otokeratin; pan keratin; Sarcolectin; tuftelin interacting protein 8; type II alpha keratin IIA; type II alpha-keratin IIC; type II cytoskeletal cochleal; Type II inner root sheath-specific keratin-K6irs2; type II mesothelial keratin K7; Type-II keratin Kb1; type-II keratin Kb12; Type-II keratin Kb2; Type-II keratin Kb3; Type-II keratin Kb35; Type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb7; Type-II keratin Kb8; Type-II keratin Kb9
Gene Aliases: 2310001L23Rik; 2310016L08Rik; 3300001P10Rik; AA960620; AI507495; AL022697; AU019895; AW108092; AW146334; BB005427; CARD2; CK-2e; CK-3; CK-4; CK-6C; CK-6E; CK-8; CK1; CK3; CK4; CK5; CK6A; CK6C; CK6D; CK7; CK8; CYK4; CYK8; D15Wsu77e; DDD; DDD1; EBS2; EGK_03684; EGK_03685; EHK; EHK1; EndoA; EPPK; HUMCYT2A; K1; K2C7; K2C8; K2E; K3; K4; K5; K6A; K6C; K6D; K6irs; K6IRS2; K7; K8; KB35; KER1; Ker2; keratin; KO; Krt-2.1; Krt-2.4; Krt-2.8; KRT1; KRT1A; KRT2; Krt2-1; Krt2-17; Krt2-2; Krt2-35; Krt2-4; Krt2-5; Krt2-6; Krt2-6a; Krt2-6c; Krt2-7; Krt2-8; KRT2A; KRT2B; KRT2E; KRT2P; KRT3; KRT4; KRT5; KRT5A; KRT6; KRT6A; KRT6B; KRT6C; KRT6D; KRT6IRS2; KRT7; KRT72; Krt72-ps; KRT75; KRT76; KRT8; Krt86; KRTA; KRTE; mK6[a]; MK6a; NEPPK; PC3; SCL; Tfip8; WSN1
UniProt ID: (Dog) Q6EIY9, (Human) P04264, (Human) P35908, (Human) P12035, (Rabbit) Q29426, (Human) P19013, (Human) P13647, (Human) P02538, (Human) P08729, (Chicken) O93532, (Human) Q14CN4, (Human) Q01546, (Human) P05787, (Mouse) P04104, (Mouse) Q3TTY5, (Mouse) P07744, (Mouse) Q922U2, (Mouse) P50446, (Mouse) Q9DCV7, (Mouse) Q6IME9, (Mouse) Q3UV17, (Mouse) P11679
Entrez Gene ID: (Dog) 444857, (Human) 3848, (Rhesus monkey) 699320, (Rhesus monkey) 699567, (Human) 3849, (Dog) 444850, (Rhesus monkey) 699194, (Human) 3850, (Dog) 100683401, (Rabbit) 100353668, (Human) 3851, (Dog) 100682832, (Rhesus monkey) 700060, (Chicken) 407779, (Dog) 100687760, (Human) 3852, (Human) 3853, (Chicken) 408041, (Dog) 477602, (Rhesus monkey) 697425, (Human) 3855, (Chicken) 395772, (Dog) 486520, (Human) 140807, (Dog) 486518, (Human) 51350, (Rabbit) 100348891, (Dog) 486513, (Rhesus monkey) 700449, (Human) 3856, (Mouse) 16678, (Mouse) 16681, (Mouse) 16682, (Mouse) 110308, (Mouse) 16687, (Mouse) 110310, (Mouse) 105866, (Mouse) 77055, (Mouse) 16691, (Rabbit) 100352658, (Rabbit) 100353415, (Rhesus monkey) 100424400, (Rhesus monkey) 100425096, (Dog) 486523, (Rhesus monkey) 718960
epithelial cell differentiation
intermediate filament cytoskeleton organization
complement activation, lectin pathway
retina homeostasis
response to oxidative stress
fibrinolysis
regulation of angiogenesis
negative regulation of inflammatory response
establishment of skin barrier
cytoskeleton organization
embryo development
negative regulation of epithelial cell proliferation
epidermis development
sebaceous gland development
viral process
keratinization
keratinocyte activation
keratinocyte proliferation
keratinocyte migration
cell morphogenesis involved in differentiation
multicellular organism development
tumor necrosis factor-mediated signaling pathway
sarcomere organization
response to hydrostatic pressure
response to other organism
cell differentiation involved in embryonic placenta development
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
morphogenesis of an epithelium
Wnt signaling pathway
wound healing
intermediate filament organization
biological_process
hemidesmosome assembly
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