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Bethyl Laboratories
Cytoskeletal Actin Recombinant Rabbit Monoclonal Antibody (BLR057F)
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FIGURE: 1 / 3
Cytoskeletal Actin Antibody (A700-057) in ICC/IF
Detection of human beta Actin by immunocytochemistry. Sample: FFPE section of HeLa cells. Antibody: Rabbit anti-beta Actin recombinant monoclonal antibody [BLR057F] (Product # A700-057) (lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Product Details
A700-057
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR057F
Immunogen
Peptide representing a region between residues 1 and 50 of human Actin Beta.
Conjugate
Form
Liquid
Concentration
10 µg/mL
Purification
purified
Storage buffer
BBS, pH 7.8 to 8.6, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; Atcb; beta actin; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; cytoskeletal gamma-actin; cytoskeleton actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; gamma actin; Gamma-actin; PS1TP5-binding protein 1
Gene Aliases: ACT; ACTB; ACTG; ACTG1; Actl; Actx; AL023024; beta-actin; BRWS1; BRWS2; DFNA20; DFNA26; E430023M04Rik; E51; HEL-176; PS1TP5BP1
UniProt ID: (Human) P60709, (Human) P63261, (Mouse) P60710, (Mouse) P63260
Entrez Gene ID: (Human) 60, (Human) 71, (Mouse) 11461, (Mouse) 11465
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
protein binding
ATP binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
ubiquitin protein ligase binding
nucleotide binding
retina homeostasis
movement of cell or subcellular component
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
sarcomere organization
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
cytoskeleton organization
substantia nigra development
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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