Proteintech

DAAM1 Polyclonal Antibody

DAAM1 Antibody in Immunocytochemistry (ICC/IF)

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DAAM1 Antibody (14876-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using DAAM1 antibody 14876-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DAAM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DAAM1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14876-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DAAM1 Fusion Protein Ag6648 (715-1068 aa encoded by BC064999)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.

Immunogen sequence: KDMLEQLLK FVPEKSDIDL LEEHKHELDR MAKADRFLFE MSRINHYQQR LQSLYFKKKF AERVAEVKPK VEAIRSGSEE VFRSGALKQL LEVVLAFGNY MNKGQRGNAY GFKISSLNKI ADTKSSIDKN ITLLHYLITI VENKYPSVLN LNEELRDIPQ AAKVNMTELD KEISTLRSGL KAVETELEYQ KSQPPQPGDK FVSVVSQFIT VASFSFSDVE DLLAEAKDLF TKAVKHFGEE AGKIQPDEFF GIFDQFLQAV SEAKQENENM RKKKEEEERR ARMEAQLKEQ RERERKMRKA KENSEESGEF DDLVSALRSG EVFDKDLSKL KRNRKRITNQ MTDSSRERPI TKLNF (715-1068 aa encoded by BC064999)

Target Information

DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The novel role of etoposide in inhibiting the migration and proliferation of small cell lung cancer and breast cancer via targeting Daam1.

Biochemical pharmacology

Yu X,Xu T,Su B,Zhou J,Xu B,Zhang Y,Zhu Y,Jiang N,He Z

Sat Apr 01 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.

Cell proliferation

Hao L,Liu Y,Yu X,Zhu Y,Zhu Y

Mon Mar 01 00:00:00 UTC 2021

Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.

Cell proliferation

Hao L,Liu Y,Yu X,Zhu Y,Zhu Y

Mon Mar 01 00:00:00 UTC 2021

Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.

Cell proliferation

Hao L,Liu Y,Yu X,Zhu Y,Zhu Y

Mon Mar 01 00:00:00 UTC 2021

Daam1 Overexpression Promotes Gastric Cancer Progression and Regulates ERK and AKT Signaling Pathways.

OncoTargets and therapy

Zhang Y,Bai X,Zhang Y,Li Y

Tue Apr 26 00:00:00 UTC 2022

Daam1 Overexpression Promotes Gastric Cancer Progression and Regulates ERK and AKT Signaling Pathways.

OncoTargets and therapy

Zhang Y,Bai X,Zhang Y,Li Y

Tue Apr 26 00:00:00 UTC 2022

The Wnt/PCP formin Daam1 drives cell-cell adhesion during nephron development.

Cell reports

Krneta-Stankic V,Corkins ME,Paulucci-Holthauzen A,Kloc M,Gladden AB,Miller RK

Tue Jul 06 00:00:00 UTC 2021

YWHAZ interacts with DAAM1 to promote cell migration in breast cancer.

Cell death discovery

Mei J,Liu Y,Yu X,Hao L,Ma T,Zhan Q,Zhang Y,Zhu Y

Fri Aug 27 00:00:00 UTC 2021

YWHAZ interacts with DAAM1 to promote cell migration in breast cancer.

Cell death discovery

Mei J,Liu Y,Yu X,Hao L,Ma T,Zhan Q,Zhang Y,Zhu Y

Fri Aug 27 00:00:00 UTC 2021

YWHAZ interacts with DAAM1 to promote cell migration in breast cancer.

Cell death discovery

Mei J,Liu Y,Yu X,Hao L,Ma T,Zhan Q,Zhang Y,Zhu Y

Fri Aug 27 00:00:00 UTC 2021

Overexpressed DAAM1 correlates with metastasis and predicts poor prognosis in breast cancer.

Pathology, research and practice

Mei J,Xu B,Hao L,Xiao Z,Liu Y,Yan T,Zhu Y

Sun Mar 01 00:00:00 UTC 2020

Overexpressed DAAM1 correlates with metastasis and predicts poor prognosis in breast cancer.

Pathology, research and practice

Mei J,Xu B,Hao L,Xiao Z,Liu Y,Yan T,Zhu Y

Sun Mar 01 00:00:00 UTC 2020

Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.

American journal of physiology. Cell physiology

He QJ,Wang P,Liu QQ,Wu QG,Li YF,Wang J,Lee SC

Wed Jan 01 00:00:00 UTC 2020

A DAAM1 3'-UTR SNP mutation regulates breast cancer metastasis through affecting miR-208a-5p-DAAM1-RhoA axis.

Cancer cell international

Mei J,Yan T,Huang Y,Xia T,Chang F,Shen S,Hao L,Chen Y,Wang Z,Jiang X,Xu B,Zhu Y

Sat Apr 09 00:00:00 UTC 2022

A DAAM1 3'-UTR SNP mutation regulates breast cancer metastasis through affecting miR-208a-5p-DAAM1-RhoA axis.

Cancer cell international

Mei J,Yan T,Huang Y,Xia T,Chang F,Shen S,Hao L,Chen Y,Wang Z,Jiang X,Xu B,Zhu Y

Sat Apr 09 00:00:00 UTC 2022

DAAM1-mediated migration and invasion of ovarian cancer cells are suppressed by miR-208a-5p.

Pathology, research and practice

Mei J,Huang Y,Hao L,Liu Y,Yan T,Qiu T,Xu R,Xu B,Xiao Z,Jiang X,Hu K,Zhu Y

Mon Jul 01 00:00:00 UTC 2019

Divergent roles of the Wnt/PCP Formin Daam1 in renal ciliogenesis.

PloS one

Corkins ME,Krneta-Stankic V,Kloc M,McCrea PD,Gladden AB,Miller RK

Wed Mar 11 00:00:00 UTC 2020

miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer.

Cellular signalling

Xiong H,Yan T,Zhang W,Shi F,Jiang X,Wang X,Li S,Chen Y,Chen C,Zhu Y

Sun Apr 01 00:00:00 UTC 2018

miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer.

Cellular signalling

Xiong H,Yan T,Zhang W,Shi F,Jiang X,Wang X,Li S,Chen Y,Chen C,Zhu Y

Sun Apr 01 00:00:00 UTC 2018

Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.

Molecular biology of the cell

Rana MK,Aloisio FM,Choi C,Barber DL

Fri Jun 15 00:00:00 UTC 2018

Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.

Molecular biology of the cell

Rana MK,Aloisio FM,Choi C,Barber DL

Fri Jun 15 00:00:00 UTC 2018

Human neuroglial cells internalize Borrelia burgdorferi by coiling phagocytosis mediated by Daam1.

PloS one