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Invitrogen
DAAM1 Polyclonal Antibody
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DAAM1 Antibody (PA5-109614) in WB
Western blot analysis of DAAM1 in extracts of various cell lines. Samples were incubated with DAAM1 Polyclonal antibody (Product # PA5-109614) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-109614
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 769-1068 of human DAAM1 (NP_001257449.1).
Conjugate
Form
Liquid
Concentration
0.84 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855025
Product Specific Information
Immunogen sequence: FKKKFAERVA EVKPKVEAIR SGSEEVFRSG ALKQLLEVVL AFGNYMNKGQ RGNAYGFKIS SLNKIADTKS SIDKNITLLH YLITIVENKY PSVLNLNEEL RDIPQAAKVN MTELDKEIST LRSGLKAVET ELEYQKSQPP QPGDKFVSVV SQFITVASFS FSDVEDLLAE AKDLFTKAVK HFGEEAGKIQ PDEFFGIFDQ FLQAVSEAKQ ENENMRKKKE EEERRARMEA QLKEQRERER KMRKAKENSE ESGEFDDLVS ALRSGEVFDK DLSKLKRNRK RITNQMTDSS RERPITKLNF
Target Information
DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Disheveled-associated activator of morphogenesis 1
Gene Aliases: DAAM1; KIAA0666
UniProt ID: (Human) Q9Y4D1, (Mouse) Q8BPM0
Entrez Gene ID: (Human) 23002, (Mouse) 208846, (Rat) 314212
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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