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          • Primary Antibodies ›
          • DACH1 Antibodies

          Proteintech

          DACH1 Polyclonal Antibody

          95 References
          View all (17) DACH1 antibodies

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          Cite DACH1 Polyclonal Antibody

          • Antibody Testing Data (14)
          DACH1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          DACH1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          DACH1 Antibody (10914-1-AP) in ICC/IF

          Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HEK-293 cells using 10914-1-AP (DACH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          DACH1 Antibody in Immunocytochemistry (ICC/IF)
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DACH1 Antibody in Western Blot (WB)
          DACH1 Antibody in Western Blot (WB)
          DACH1 Antibody in Western Blot (WB)
          DACH1 Antibody in Immunoprecipitation (IP)

          Product Details

          10914-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 25 publications 25 publications

          Immunohistochemistry (IHC)

          -
          View 28 publications 28 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          View 33 publications 33 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 1 publication 1 publication

          ChIP assay (ChIP)

          Assay-dependent
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications

          Not applicable (N/A)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          DACH1 Fusion Protein Ag1354 (176-521 aa encoded by BC021219)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.14 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

          Immunogen sequence: QHGADSENG DMNSSVGLEL PFMMMPHPLI PVSLPPASVT MAMSQMNHLS TIANMAAAAQ VQSPPSRVET SVIKERVPDS PSPAPSLEEG RRPGSHPSSH RSSSVSSSPA RTESSSDRIP VHQNGLSMNQ MLMGLSPNVL PGPKEGDLAG HDMGHESKRM HIEKDETPLS TPTARDSLDK LSLTGHGQPL PPGFPSPFLF PDGLSSIETL LTNIQGLLKV AIDNARAQEK QVQLEKTELK MDFLRERELR ETLEKQLAME QKNRAIVQKR LKKEKKAKRK LQEALEFETK RREQAEQTLK QAASTDSLRV LNDSLTPEIE ADRSGGRTDA ERTIQDGRLY LKTTVMY (176-521 aa encoded by BC021219 )

          Target Information

          Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: dac homolog; Dach1; dachshund family transcription factor 1; Dachshund homolog 1; FLJ10138; OTTHUMP00000040839; RP11-512J14.1

          View more View less

          Gene Aliases: Dac; DACH; DACH1

          View more View less

          UniProt ID: (Human) Q9UI36, (Mouse) Q9QYB2

          View more View less

          Entrez Gene ID: (Human) 1602, (Rat) 306096, (Mouse) 13134

          View more View less

          Function(s)
          transcription factor activity, RNA polymerase II core promoter sequence-specific binding involved in preinitiation complex assembly transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding protein binding transcription factor activity, sequence-specific DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter suckling behavior multicellular organism development respiratory gaseous exchange cell proliferation negative regulation of transcription by competitive promoter binding negative regulation of cell migration regulation of nuclear cell cycle DNA replication negative regulation of transcription, DNA-templated development of primary female sexual characteristics negative regulation of fibroblast proliferation RNA polymerase II transcriptional preinitiation complex assembly negative regulation of cell proliferation involved in contact inhibition negative regulation of DNA biosynthetic process regulation of transcription, DNA-templated transcription, DNA-templated transcription from RNA polymerase II promoter
          It has to be done as per old AB suggested Products section.
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