Proteintech

DAPK1 Polyclonal Antibody

DAPK1 Antibody in Immunocytochemistry (ICC/IF)

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DAPK1 Antibody (25136-1-AP) in ICC/IF

Immunofluorescent analysis of A549 cells using 25136-1-AP (DAPK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DAPK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

25136-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DAPK1 Fusion Protein Ag18387 (1081-1410 aa encoded by BC113660)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.01 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LLQILDAMD ICARDLSSGT MVDVPALIKT DNLHRSWADE EDEVMVYGGV RIVPVEHLTP FPCGIFHKVQ VNLCRWIHQQ STEGDADIRL WVNGCKLANR GAELLVLLVN HGQGIEVQVR GLETEKIKCC LLLDSVCSTI ENVMATTLPG LLTVKHYLSP QQLREHHEPV MIYQPRDFFR AQTLKETSLT NTMGGYKESF SSIMCFGCHD VYSQASLGMD IHASDLNLLT RRKLSRLLDP PDPLGKDWCL LAMNLGLPDL VAKYNTSNGA PKDFLPSPLH ALLREWTTYP ESTVGTLMSK LRELGRRDAA DFLLKASSVF KINLDGNGQE A (1081-1410 aa encoded by BC113660)

Target Information

DAPK1 is a large, calmodulin-dependent kinase involved in gamma-interferon induced programmed cell death and is a potential tumor suppressor. DAPK1 is involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and authophagy. It regulates both type I apoptotic and type II autophagic cell death signal, depending on the cellular setting. Type I apoptotic cell death is caspase-dependent, type II authophagic cell death is caspase-independent. DAPK1 phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. It also phosphorylates TPM1, STX1A, PRKD1, BECN1, TSC2, RPS6, MYL9, and DAPK3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long non-coding RNA nuclear paraspeckle assembly transcript 1 downregulation protects lens epithelial cells from oxidative stress-induced apoptosis by regulating the microRNA-124-3p/death-associated protein kinase 1 axis in age-related cataract.

International ophthalmology

Guo X,Li C,Wang Y,Jiang C,Yang L

Fri Sep 01 00:00:00 UTC 2023

Sanggenon C inhibits cell proliferation and induces apoptosis by regulating the MIB1/DAPK1 axis in glioblastoma.

MedComm

Chang H,Hou J,Shao Y,Xu M,Weng X,Du Y,Shi J,Zhang L,Cui H

Tue Aug 01 00:00:00 UTC 2023

Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage.

Life sciences

Hu BC,Zhu JW,Wu GH,Cai JJ,Yang X,Shao ZQ,Zheng Y,Lai JM,Shen Y,Yang XH,Liu JQ,Sun RH,Zhu HP,Ye XM,Mo SJ

Thu Jun 01 00:00:00 UTC 2023

Yifei sanjie Pills Alleviate Chemotherapy-Related Fatigue by Reducing Skeletal Muscle Injury and Inhibiting Tumor Growth in Lung Cancer Mice.

Evidence-based complementary and alternative medicine : eCAM

Wu Y,Pi D,Chen Y,Zuo Q,Lin L,Ouyang M

Wed Sep 07 00:00:00 UTC 2022

Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.

Stem cell research & therapy

Chu M,Wan H,Zhang X

Thu Jan 28 00:00:00 UTC 2021

Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.

Stem cells and development

Nong S,Wei Z,Wang Z,Ma L,Guan Y,Ni J

Wed Sep 15 00:00:00 UTC 2021

Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.

Stem cells and development

Nong S,Wei Z,Wang Z,Ma L,Guan Y,Ni J

Wed Sep 15 00:00:00 UTC 2021

Death-Associated Protein Kinase 1 Regulates Oxidative Stress in Cardiac Ischemia Reperfusion Injury.

Cells, tissues, organs

Li W,Yu W,Xu W,Xiong J,Zhong X,Hu S,Yu J

Mon Jan 17 00:00:00 UTC 2022

Comparison of the Effects of Nonprotein and Protein Nitrogen on Apoptosis and Autophagy of Rumen Epithelial Cells in Goats.

Animals : an open access journal from MDPI

Kong Z,Zhou C,Kang J,Tan Z

Mon Nov 09 00:00:00 UTC 2020

Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma.

Aging

Song Z,Li Z,Han W,Zhu C,Lou N,Li X,Luo G,Peng S,Li G,Zhao Y,Guo Y

Mon Nov 16 00:00:00 UTC 2020

Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma.

Aging

Song Z,Li Z,Han W,Zhu C,Lou N,Li X,Luo G,Peng S,Li G,Zhao Y,Guo Y

Mon Nov 16 00:00:00 UTC 2020

Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma.

Aging

Song Z,Li Z,Han W,Zhu C,Lou N,Li X,Luo G,Peng S,Li G,Zhao Y,Guo Y

Mon Nov 16 00:00:00 UTC 2020

Oxytocin Alleviates MPTP-Induced Neurotoxicity in Mice by Targeting MicroRNA-26a/Death-Associated Protein Kinase 1 Pathway.

Journal of Alzheimer's disease : JAD

Almansoub HAMM,Tang H,Wu Y,Wang DQ,Mahaman YAR,Salissou MTM,Lu Y,Hu F,Zhou LT,Almansob YAM,Liu D

Mon Apr 19 00:00:00 UTC 2021

Central Regulatory Role for SIN1 in Interferon γ (IFNγ) Signaling and Generation of Biological Responses.

The Journal of biological chemistry

Kroczynska B,Blyth GT,Rafidi RL,Majchrzak-Kita B,Xu L,Saleiro D,Kosciuczuk EM,Jemielity J,Su B,Altman JK,Eklund EA,Fish EN,Platanias LC

Fri Mar 17 00:00:00 UTC 2017

Central Regulatory Role for SIN1 in Interferon γ (IFNγ) Signaling and Generation of Biological Responses.

The Journal of biological chemistry

Kroczynska B,Blyth GT,Rafidi RL,Majchrzak-Kita B,Xu L,Saleiro D,Kosciuczuk EM,Jemielity J,Su B,Altman JK,Eklund EA,Fish EN,Platanias LC

Fri Mar 17 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: DAP kinase 1; Death-associated protein kinase 1; DKFZp781I035; OTTHUMP00000216193

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Gene Aliases: DAPK; DAPK1

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UniProt ID: (Human) P53355

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Entrez Gene ID: (Human) 1612

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Function(s)

protein kinase activity protein serine/threonine kinase activity calmodulin-dependent protein kinase activity protein binding calmodulin binding ATP binding GTP binding syntaxin-1 binding identical protein binding

Process(es)

protein phosphorylation apoptotic process extrinsic apoptotic signaling pathway via death domain receptors regulation of autophagy negative regulation of translation intracellular signal transduction regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process protein autophosphorylation cellular response to interferon-gamma apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of N-methyl-D-aspartate selective glutamate receptor activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DAPK1 Polyclonal Antibody

DAPK1 Antibody (25136-1-AP) in ICC/IF

Product Details

25136-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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