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Invitrogen
DARC Polyclonal Antibody
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DARC Antibody (PA5-47861) in IHC (F)
Immunohistochemical analysis of DARC in perfusion fixed frozen sections of mouse skin. Samples were incubated with DARC polyclonal antibody (Product # PA5-47861) using a dilution of 0.5 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the hair follicle.
Product Details
PA5-47861
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse DARC Met1-Pro61, Ala115-Cys127, Ser186-Lys205, Tyr264-Asn285
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576815
Product Specific Information
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
ACKR1 or DARC is an atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades resulting instead in chemokine sequestration, degradation, or transcytosis. ACKR1 is also known as an interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. It has a promiscuous chemokine-binding profile by interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. ACKR1 also acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and for the malaria parasites P.vivax and P.knowlesi.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Atypical chemokine receptor 1; CD234; CD234 antigen; CTA-134P22.3; Duffy Antigen; Duffy antigen receptor for chemokines; Duffy antigen/chemokine receptor; Duffy blood group, atypical chemokine receptor; Duffy blood group, chemokine receptor; Fy Antigen; FYDuffy blood group; glycoprotein D; GPDWBCQ1; HGNC:4035; OTTHUMP00000060081
Gene Aliases: AA162249; Ackr1; CCBP1; CD234; Darc; Dfy; ESTM35; FY; GPD
UniProt ID: (Mouse) Q9QUI6
Entrez Gene ID: (Rat) 689105, (Mouse) 13349
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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