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DARS2 Antibody (PA5-100238) in ICC/IF
Immunofluorescent analysis of DARS2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DARS2 polyclonal antibody (Product # PA5-100238) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100238
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DARS2(Accession Q6PI48), corresponding to amino acid residues Q245-S295.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815768
Product Specific Information
Antibody detects endogenous levels of total DARS2.
Target Information
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aspartate tRNA ligase 2, mitochondrial; Aspartate--tRNA ligase, mitochondrial; Aspartyl-tRNA synthetase; aspartyl-tRNA synthetase, mitochondrial; AspRS
Gene Aliases: 5830468K18Rik; ASPRS; DARS2; LBSL; MT-ASPRS; RGD1308286
UniProt ID: (Human) Q6PI48, (Rat) Q3KRD0, (Mouse) Q8BIP0
Entrez Gene ID: (Human) 55157, (Rat) 304919, (Mouse) 226539
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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