Rockland

DAXX Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-966

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200-1:2,000
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ELISA (ELISA)

1:7,000-1:45,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-275 of human DAXX protein.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.06 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against human DAXX protein. A BLAST analysis was used to suggest cross reactivity with DAXX protein from human, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Target Information

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CENP-C binding protein; DADB-159G18.9; Daxx; Death domain-associated protein 6; death-associated protein 6; death-domain associated protein; EAP1; ETS1-associated protein 1; Fas death domain-associated protein; Fas-binding protein; hDaxx; MGC126245; MGC126246; SMIM40

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Gene Aliases: BING2; DAP6; DAXX; EAP1

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UniProt ID: (Human) Q9UER7, (Mouse) O35613

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Entrez Gene ID: (Human) 1616, (Mouse) 13163, (Rat) 140926

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Function(s)
p53 binding transcription corepressor activity receptor signaling protein activity protein binding transcription factor binding enzyme binding protein kinase binding protein kinase activator activity heat shock protein binding ubiquitin protein ligase binding histone binding protein N-terminus binding androgen receptor binding transcription coactivator activity protein homodimerization activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis positive regulation of protein phosphorylation nucleosome assembly chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process negative regulation of myotube differentiation chromatin modification androgen receptor signaling pathway PML body organization regulation of protein ubiquitination positive regulation of histone phosphorylation positive regulation of apoptotic process positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter apoptotic signaling pathway positive regulation of neuron death negative regulation of nucleic acid-templated transcription positive regulation of apoptotic signaling pathway response to metal ion cellular response to tumor necrosis factor cellular response to anoxia activation of JUN kinase activity extrinsic apoptotic signaling pathway via death domain receptors viral process covalent chromatin modification
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