Invitrogen

DAXX Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-79137

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
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Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

0.5-1 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ChIP assay (ChIP)

2.5 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Daxx recombinant protein (Position: K56-R345).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746253

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell, rat PC-12 whole cell, mouse thymus tissue. IHC: rat intestine tissue, human intestinal cancer tissue. ICC/IF: U20S cell, Hela cell, SMMC-7721 cell, A549 cell. Flow: 293T cell.

Target Information

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CENP-C binding protein; DADB-159G18.9; Daxx; Death domain-associated protein 6; death-associated protein 6; death-domain associated protein; EAP1; ETS1-associated protein 1; Fas death domain-associated protein; Fas-binding protein; hDaxx; MGC126245; MGC126246; SMIM40

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Gene Aliases: BING2; DAP6; DAXX; EAP1

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UniProt ID: (Human) Q9UER7, (Mouse) O35613

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Entrez Gene ID: (Human) 1616, (Mouse) 13163, (Rat) 140926

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Function(s)
p53 binding transcription corepressor activity receptor signaling protein activity protein binding transcription factor binding enzyme binding protein kinase binding protein kinase activator activity heat shock protein binding ubiquitin protein ligase binding histone binding protein N-terminus binding androgen receptor binding transcription coactivator activity protein homodimerization activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis positive regulation of protein phosphorylation nucleosome assembly chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process negative regulation of myotube differentiation chromatin modification androgen receptor signaling pathway PML body organization regulation of protein ubiquitination positive regulation of histone phosphorylation positive regulation of apoptotic process positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter apoptotic signaling pathway positive regulation of neuron death negative regulation of nucleic acid-templated transcription positive regulation of apoptotic signaling pathway response to metal ion cellular response to tumor necrosis factor cellular response to anoxia activation of JUN kinase activity extrinsic apoptotic signaling pathway via death domain receptors viral process covalent chromatin modification
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