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Product Details
H00010926-M01
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
6G9
Immunogen
DBF4 (NP_006707, 2 a.a. approximately 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Product may be used with Western Blot (Transfected lysate).
Immunogen sequence: NSGAMRIHSK GHFQGGIQVK NEKNRPSLKS LKTDNRPEKS KCKPLWGKVF YLDLPSVTIS EKLQKDIKDL GGRVEEFLSK DISYLISNKK EAKFAQT
Target Information
Activator of S phase kinase (ASK, DBF4) is a novel regulatory subunit of the human Cdc7 kinase complex that plays a pivotal role in G1/S cell cycle transition in mammalian cells. The increase in Cdc7 kinase activity at the G1/S boundary is at least partly accounted for by the elevated expression of ASK in late G1. ASK interacts with replication origins in vivo, suggesting that Cdc7 may trigger S phase by directly activating the replication initiation complexes assembled at the origins. Human ASK protein levels are regulated during the cell cycle with a pattern that matches that of human Cdc7 protein kinase activity. Human Cdc7-ASK kinase is directly involved in regulating the initiation of DNA replication by targeting MCM2 protein in mammalian cells. Nuclear localization and chromatin binding of endogenous huCdc7 and GFP-ASK is presumably imported into nuclei through its two nuclear localization signals at telophase, it may require additional signals for chromatin binding, the level of which increases at late G1 ohase. A functional link may exist between menin, a tumor suppressor protein mutated in multiple endocrine neoplasia type I (MEN1), and ASK in the regulation of cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator of S phase kinase; Chiffon homolog A; DBF4 homolog; DBF4 zinc finger A; DBF4-type zinc finger-containing protein 1; Protein DBF4 homolog A; zinc finger, DBF-type containing 1
Gene Aliases: ASK; CHIF; DBF4; DBF4A; ZDBF1
UniProt ID: (Human) Q9UBU7
Entrez Gene ID: (Human) 10926
Performance Guarantee
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