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Invitrogen
DCI Polyclonal Antibody
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FIGURE: 1 / 6
DCI Antibody (PA5-79184) in ICC/IF
Immunocytochemistry analysis of DCI using anti-DCI antibody (Product # PA5-79184) . DCI was detected in a section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DCI antibody (Product # PA5-79184) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter... View More
Product Details
PA5-79184
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DCI (272-290aa ADVQNFVSFISKDSIQKSL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746300
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Liver Tissue, Human Placenta Tissue, A549 whole cell, SMMC whole cell, COLO320 whole cell, HELA whole cell, HT1080 whole cell. IHC: human mammary cancer tissue. ICC/IF: A549 cell, A549 cell, human Hela cell.
Target Information
Protein names: Enoyl-CoA delta isomerase 1, mitochondrial; 3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; Short name=D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase. Gene names: ECI1, DCI. Function: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Catalytic activity: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. Pathway: Lipid metabolism; fatty acid beta-oxidation. Subunit structure: Homotrimer. Subcellular location: Mitochondrion matrix.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3,2 trans-enoyl-Coenyme A isomerase; 3,2 trans-enoyl-Coenzyme A isomerase; 3,2-trans-enoyl-CoA isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; ACBD2; acetylene-allene isomerase; D3,D2-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; dodecenoyl-CoA de; dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase); Dodecenoyl-CoA isomerase; dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenyme A isomerase); dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase); DRS 1; DRS1; Enoyl-CoA delta isomerase 1, mitochondrial; enoyl-Coenzyme A delta isomerase 1; HCA88; Peroxisomal 3
Gene Aliases: DCI; eci; ECI1
UniProt ID: (Human) P42126, (Mouse) P42125
Entrez Gene ID: (Human) 1632, (Rat) 29740, (Mouse) 13177
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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