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DCTN3 Antibody (BS-7746R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with DCTN3 Polyclonal Antibody (bs-7746R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product Details
BS-7746R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human DCTN3, amino acids 41-150.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DCTN3; dynactin 3 (p22); Dynactin complex subunit 22 kDa subunit; dynactin light chain; dynactin light chain (P24); Dynactin light chain p24; Dynactin subunit 3; MGC111190; p22; p24 dynactin light chain
Gene Aliases: DCTN-22; DCTN22; DCTN3; p24
UniProt ID: (Human) O75935, (Mouse) Q9Z0Y1
Entrez Gene ID: (Human) 11258, (Rat) 362504, (Mouse) 53598
Performance Guarantee
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