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          • Primary Antibodies ›
          • DDAH2 Antibodies

          Invitrogen

          DDAH2 Recombinant Rabbit Monoclonal Antibody (5M4Y4)

          View all (16) DDAH2 antibodies

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          Cite DDAH2 Recombinant Rabbit Monoclonal Antibody (5M4Y4)

          • Antibody Testing Data (1)
          DDAH2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          DDAH2 Antibody in Western Blot (WB)
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          FIGURE: 1 / 1

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          DDAH2 Antibody (MA5-35453) in WB

          Western blot analysis of DDAH2 in extracts of various cell lines. Samples were incubated with DDAH2 Monoclonal antibody (Product # MA5-35453) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          DDAH2 Antibody in Western Blot (WB)

          Product Details

          MA5-35453

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          5M4Y4

          Immunogen

          A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDAH2 (O95865)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.05 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2849354

          Product Specific Information

          Immunogen sequence: MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV

          Target Information

          This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Clone 7u; DADB-110M10.5; DDAH-2; DDAHII; dimethylargininase-2; dimethylarginine dimethylaminohydrolase II; epididymis secretory protein Li 277; Inactive N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; OTTHUMP00000029307; OTTHUMP00000062666; OTTHUMP00000174406; Protein G6a; Putative hydrolase DDAH2; S-phase protein; testis tissue sperm-binding protein Li 54e

          View more View less

          Gene Aliases: 1110003M04Rik; AU019324; AW413173; DDAH; DDAH-2; DDAH2; DDAHII; G6A; HEL-S-277; NG30

          View more View less

          UniProt ID: (Human) O95865, (Mouse) Q99LD8, (Rat) Q6MG60

          View more View less

          Entrez Gene ID: (Human) 23564, (Mouse) 51793, (Rat) 294239

          View more View less

          Function(s)
          catalytic activity protein binding dimethylargininase activity amino acid binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines
          Process(es)
          citrulline metabolic process arginine metabolic process arginine catabolic process nitric oxide biosynthetic process nitric oxide mediated signal transduction negative regulation of apoptotic process positive regulation of nitric oxide biosynthetic process regulation of nitric-oxide synthase activity
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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