Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
DDI1 Antibody (PA5-89849) in WB
Western blot analysis of DDI1 in extracts of Mouse testis. Samples were incubated with DDI1 Polyclonal antibody (Product # PA5-89849) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89849
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 97-396 of human DDI1 (NP_0010017111)
Conjugate
Form
Liquid
Concentration
2.16 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805800
Product Specific Information
Immunogen sequence: SGIAVPGTSS SRPQHPGQQQ QRTPAAQRSQ GLASGEKVAG LQGLGSPALI RSMLLSNPHD LSLLKERNPP LAEALLSGSL ETFSQVLMEQ QREKALREQE RLRLYTADPL DREAQAKIEE EIRQQNIEEN MNIAIEEAPE SFGQVTMLYI NCKVNGHPLK AFVDSGAQMT IMSQACAERC NIMRLVDRRW AGVAKGVGTQ RIIGRVHLAQ IQIEGDFLQC SFSILEDQPM DMLLGLDMLR RHQCSIDLKK NVLVIGTTGT QTYFLPEGEL PLCSRMVSGQ DESSDKEITH SVMDSGRKEH; Positive Samples: Mouse testis
Target Information
Ddi1 (DNA-damage inducible 1)appears to release substrate from an ubiquitination complex, making the substrate available for deubiquitination to facilitate proteasomal degradation. Ddi1 could be a potential selective target for lung cancer treatment. Ddi1 runs as a doublet of 54/56 kDa on acrylamide gels besides the calculated MW 44 kDa.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DDI1, DNA-damage inducible 1, homolog 1; DNA-damage inducible 1 homolog 1; DNA-damage inducible protein 1; Protein DDI1 homolog 1
Gene Aliases: 1700011N24Rik; DDI1
UniProt ID: (Human) Q8WTU0, (Mouse) Q9DAF3
Entrez Gene ID: (Human) 414301, (Mouse) 71829
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support