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OriGene
DDK Monoclonal Antibody (OTI4C5 (formerly 4C5)), TrueMAB™
FIGURE: 1 / 17
DDK Antibody (TA50011-100) in ICC/IF
Immunofluorescence staining for F-actin (green) and merlin mutants (red) in CH157 NM cells transfected with wild-type merlin, merlin mutants, as well as empty vector (blank). The nuclei of cells were counterstained with Hoechst (blue). Arrows point to F-actin stress fibers and arrowheads point to mutant merlin.to right) and negative control vector (lane 1) using anti-HA tag rabbit monoclonal antibody (TA591010) at 1:2000 dilution. (https://doi.org/10.1073/pnas.1102198108)
Product Details
TA50011-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI4C5 (formerly 4C5)
Immunogen
Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Synthetic peptide sequence is DYKDDDDK
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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