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Invitrogen
DDOST Polyclonal Antibody
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DDOST Antibody (PA5-97181) in WB
Western blot analysis of DDOST in extracts of various cell lines. Samples were incubated with DDOST Polyclonal antibody (Product # PA5-97181) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-97181
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human DDOST (NP_0052072)
Conjugate
Form
Liquid
Concentration
2.88 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808983
Product Specific Information
Immunogen sequence: VCASGPRTLV LLDNLNVRET HSLFFRSLKD RGFELTFKTA DDPSLSLIKY GEFLYDNLII FSPSVEDFGG NINVETISAF IDGGGSVLVA ASSDIGDPLR ELGSECGIEF DEEKTAVIDH HNYDISDLGQ HTLIVADTEN LLKAPTIVGK SSLNPILFRG VGMVADPDNP LVLDILTGSS TSYSFFPDKP ITQYPHAVGK NTLLIAGLQA RNNARVIFSG SLDFFSDSFF NSAVQKAAPG SQRYSQTGNY ELAVALSRWV F; Positive Samples: U-87MG, HT-29, HepG2, A-549, Mouse kidney, Mouse liver, Mouse brain, Rat testis; Cellular Location: Endoplasmic reticulum membrane, Single-pass type I membrane protein
Target Information
This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation end products (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: advanced glycation end-product receptor 1; advanced glycation endproduct receptor 1; AGE-R1; DDOST 48 kDa subunit; dolichyl-di-phosphooligosaccharide-protein glycotransferase; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic); dolichyl-diphosphooligosaccharide-protein glycotransferase; MGC2191; oligosaccharyl transferase 48 kDa subunit; oligosaccharyltransferase 48 kDa subunit; oligosaccharyltransferase subunit 48
Gene Aliases: AGER1; CDG1R; DDOST; KIAA0115; OK/SW-cl.45; OKSWcl45; OST; OST48; WBP1
UniProt ID: (Human) P39656, (Mouse) O54734, (Rat) Q641Y0
Entrez Gene ID: (Human) 1650, (Mouse) 13200, (Rat) 313648
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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