Proteintech

DDRGK1 Polyclonal Antibody

DDRGK1 Antibody in Immunocytochemistry (ICC/IF)

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DDRGK1 Antibody (21445-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 21445-1-AP (DDRGK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DDRGK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

21445-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Goat, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DDRGK1 Fusion Protein Ag13742 (1-314 aa encoded by BC000643)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.

Immunogen sequence: MVAPVWYLV AAALLVGFIL FLTRSRGRAA SAGQEPLHNE ELAGAGRVAQ PGPLEPEEPR AGGRPRRRRD LGSRLQAQRR AQRVAWAEAD ENEEEAVILA QEEEGVEKPA ETHLSGKIGA KKLRKLEEKQ ARKAQREAEE AEREERKRLE SQREAEWKKE EERLRLEEEQ KEEEERKARE EQAQREHEEY LKLKEAFVVE EEGVGETMTE EQSQSFLTEF INYIKQSKVV LLEDLASQVG LRTQDTINRI QDLLAEGTIT GVIDDRGKFI YITPEELAAV ANFIRQRGRV SIAELAQASN SLIAWGRESP AQAPA (1-314 aa encoded by BC000643)

Target Information

Protein which interacts with the E3 UFM1-protein ligase UFL1 and one of its substrates TRIP4 and is required for TRIP4 ufmylation. Through TRIP4 ufmylation may regulate nuclear receptors-mediated transcription. May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIA, the inhibitor of NF-kappa-B. May also play a role in the cellular response to endoplasmic reticulum stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A neuroprotective role of Ufmylation through Atg9 in the aging brain of Drosophila.

Cellular and molecular life sciences : CMLS

Li H,Yu Z,Niu Z,Cheng Y,Wei Z,Cai Y,Ma F,Hu L,Zhu J,Zhang W

Sat Apr 22 00:00:00 UTC 2023

DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang X,Zhou T,Yang X,Cao X,Jin G,Zhang P,Guo J,Rong K,Li B,Hu Y,Liu K,Ma P,Qin A,Zhao J

Mon May 01 00:00:00 UTC 2023

DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang X,Zhou T,Yang X,Cao X,Jin G,Zhang P,Guo J,Rong K,Li B,Hu Y,Liu K,Ma P,Qin A,Zhao J

Mon May 01 00:00:00 UTC 2023

DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang X,Zhou T,Yang X,Cao X,Jin G,Zhang P,Guo J,Rong K,Li B,Hu Y,Liu K,Ma P,Qin A,Zhao J

Mon May 01 00:00:00 UTC 2023

Excessive ER-phagy contributes to ochratoxin A-induced apoptosis.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Deng H,Chen W,Zhang B,Zhang Y,Han L,Zhang Q,Yao S,Wang H,Shen XL

Thu Jun 01 00:00:00 UTC 2023

A non-canonical scaffold-type E3 ligase complex mediates protein UFMylation.

The EMBO journal

Peter JJ,Magnussen HM,DaRosa PA,Millrine D,Matthews SP,Lamoliatte F,Sundaramoorthy R,Kopito RR,Kulathu Y

Wed Nov 02 00:00:00 UTC 2022

The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.

Nature communications

Ishimura R,El-Gowily AH,Noshiro D,Komatsu-Hirota S,Ono Y,Shindo M,Hatta T,Abe M,Uemura T,Lee-Okada HC,Mohamed TM,Yokomizo T,Ueno T,Sakimura K,Natsume T,Sorimachi H,Inada T,Waguri S,Noda NN,Komatsu M

Wed Dec 21 00:00:00 UTC 2022

Deficiency of Murine UFM1-Specific E3 Ligase Causes Microcephaly and Inflammation.

Molecular neurobiology

Zhang J,Zhu H,Liu S,Quintero M,Zhu T,Xu R,Cai Y,Han Y,Li H

Sat Oct 01 00:00:00 UTC 2022

Ufbp1, a Key Player of Ufm1 Conjugation System, Protects Against Ketosis-Induced Liver Injury via Suppressing Smad3 Activation.

Frontiers in cell and developmental biology

Chen F,Sheng L,Xu C,Li J,Ali I,Li H,Cai Y

Tue Jul 27 00:00:00 UTC 2021

Ufbp1, a Key Player of Ufm1 Conjugation System, Protects Against Ketosis-Induced Liver Injury via Suppressing Smad3 Activation.

Frontiers in cell and developmental biology

Chen F,Sheng L,Xu C,Li J,Ali I,Li H,Cai Y

Tue Jul 27 00:00:00 UTC 2021

A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy.

Genetics in medicine : official journal of the American College of Medical Genetics

Ni M,Afroze B,Xing C,Pan C,Shao Y,Cai L,Cantarel BL,Pei J,Grishin NV,Hewson S,Knight D,Mahida S,Michel D,Tarnopolsky M,Poduri A,Rotenberg A,Sondheimer N,DeBerardinis RJ

Sat May 01 00:00:00 UTC 2021

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Cell death & disease

Cao Y,Li R,Shen M,Li C,Zou Y,Jiang Q,Liu S,Lu C,Li H,Liu H,Cai Y

Tue Apr 20 00:00:00 UTC 2021

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Cell death & disease

Cao Y,Li R,Shen M,Li C,Zou Y,Jiang Q,Liu S,Lu C,Li H,Liu H,Cai Y

Tue Apr 20 00:00:00 UTC 2021

Ufmylation regulates granulosa cell apoptosis via ER stress but not oxidative stress during goat follicular atresia.

Theriogenology

Zhang X,Yu T,Guo X,Zhang R,Jia Y,Shang C,Wang A,Jin Y,Lin P

Thu Jul 15 00:00:00 UTC 2021

Ufmylation regulates granulosa cell apoptosis via ER stress but not oxidative stress during goat follicular atresia.

Theriogenology

Zhang X,Yu T,Guo X,Zhang R,Jia Y,Shang C,Wang A,Jin Y,Lin P

Thu Jul 15 00:00:00 UTC 2021

Ufmylation regulates granulosa cell apoptosis via ER stress but not oxidative stress during goat follicular atresia.

Theriogenology

Zhang X,Yu T,Guo X,Zhang R,Jia Y,Shang C,Wang A,Jin Y,Lin P

Thu Jul 15 00:00:00 UTC 2021

Cdk5rap3 is essential for intestinal Paneth cell development and maintenance.

Cell death & disease

Quintero M,Liu S,Xia Y,Huang Y,Zou Y,Li G,Hu L,Singh N,Blumberg R,Cai Y,Xu H,Li H

Wed Jan 27 00:00:00 UTC 2021

A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Cell

Liang JR,Lingeman E,Luong T,Ahmed S,Muhar M,Nguyen T,Olzmann JA,Corn JE

Thu Mar 19 00:00:00 UTC 2020

UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis.

Cell research

Wang L,Xu Y,Rogers H,Saidi L,Noguchi CT,Li H,Yewdell JW,Guydosh NR,Ye Y

Wed Jan 01 00:00:00 UTC 2020

UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination.

Nature cell biology

Liu J,Guan D,Dong M,Yang J,Wei H,Liang Q,Song L,Xu L,Bai J,Liu C,Mao J,Zhang Q,Zhou J,Wu X,Wang M,Cong YS

Tue Sep 01 00:00:00 UTC 2020

UFMylation is associated with LPS-induced inflammatory response in goat endometrial epithelial cells.

Reproduction in domestic animals = Zuchthygiene

Xiao J,Liu S,Yu T,Zhang R,Guo X,Jia Y,Shang C,Wang A,Jin Y,Lin P

Tue Dec 01 00:00:00 UTC 2020

UFMylation is associated with LPS-induced inflammatory response in goat endometrial epithelial cells.

Reproduction in domestic animals = Zuchthygiene

Xiao J,Liu S,Yu T,Zhang R,Guo X,Jia Y,Shang C,Wang A,Jin Y,Lin P

Tue Dec 01 00:00:00 UTC 2020

UFMylation is associated with LPS-induced inflammatory response in goat endometrial epithelial cells.

Reproduction in domestic animals = Zuchthygiene

Xiao J,Liu S,Yu T,Zhang R,Guo X,Jia Y,Shang C,Wang A,Jin Y,Lin P

Tue Dec 01 00:00:00 UTC 2020

UFMylation is associated with LPS-induced inflammatory response in goat endometrial epithelial cells.

Reproduction in domestic animals = Zuchthygiene

Xiao J,Liu S,Yu T,Zhang R,Guo X,Jia Y,Shang C,Wang A,Jin Y,Lin P

Tue Dec 01 00:00:00 UTC 2020

Salubrinal, a novel inhibitor of eIF-2α dephosphorylation, promotes erythropoiesis at early stage targeted by ufmylation pathway.

Journal of cellular physiology

Chen F,Xing C,Zhang W,Li J,Hu T,Li L,Li H,Cai Y

Thu Aug 01 00:00:00 UTC 2019

Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation.

Cell discovery

Cai Y,Zhu G,Liu S,Pan Z,Quintero M,Poole CJ,Lu C,Zhu H,Islam B,Riggelen JV,Browning D,Liu K,Blumberg R,Singh N,Li H

Thu Oct 01 00:00:00 UTC 2020

Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation.

Cell discovery

Cai Y,Zhu G,Liu S,Pan Z,Quintero M,Poole CJ,Lu C,Zhu H,Islam B,Riggelen JV,Browning D,Liu K,Blumberg R,Singh N,Li H

Thu Oct 01 00:00:00 UTC 2020

Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation.

Cell discovery

Cai Y,Zhu G,Liu S,Pan Z,Quintero M,Poole CJ,Lu C,Zhu H,Islam B,Riggelen JV,Browning D,Liu K,Blumberg R,Singh N,Li H

Thu Oct 01 00:00:00 UTC 2020

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Wed Mar 06 00:00:00 UTC 2019

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Wed Mar 06 00:00:00 UTC 2019

Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer.

World journal of gastroenterology

Lin JX,Xie XS,Weng XF,Zheng CH,Xie JW,Wang JB,Lu J,Chen QY,Cao LL,Lin M,Tu RH,Li P,Huang CM

Fri Sep 14 00:00:00 UTC 2018

Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer.

World journal of gastroenterology

Lin JX,Xie XS,Weng XF,Zheng CH,Xie JW,Wang JB,Lu J,Chen QY,Cao LL,Lin M,Tu RH,Li P,Huang CM

Fri Sep 14 00:00:00 UTC 2018

Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer.

World journal of gastroenterology

Lin JX,Xie XS,Weng XF,Zheng CH,Xie JW,Wang JB,Lu J,Chen QY,Cao LL,Lin M,Tu RH,Li P,Huang CM

Fri Sep 14 00:00:00 UTC 2018

Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer.

World journal of gastroenterology

Lin JX,Xie XS,Weng XF,Zheng CH,Xie JW,Wang JB,Lu J,Chen QY,Cao LL,Lin M,Tu RH,Li P,Huang CM

Fri Sep 14 00:00:00 UTC 2018

Proteomic and Biochemical Analyses Reveal a Novel Mechanism for Promoting Protein Ubiquitination and Degradation by UFBP1, a Key Component of Ufmylation.

Journal of proteome research

Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

Fri Apr 06 00:00:00 UTC 2018

Proteomic and Biochemical Analyses Reveal a Novel Mechanism for Promoting Protein Ubiquitination and Degradation by UFBP1, a Key Component of Ufmylation.

Journal of proteome research

Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

Fri Apr 06 00:00:00 UTC 2018

Proteomic and Biochemical Analyses Reveal a Novel Mechanism for Promoting Protein Ubiquitination and Degradation by UFBP1, a Key Component of Ufmylation.

Journal of proteome research

Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

Fri Apr 06 00:00:00 UTC 2018

Proteomic and Biochemical Analyses Reveal a Novel Mechanism for Promoting Protein Ubiquitination and Degradation by UFBP1, a Key Component of Ufmylation.

Journal of proteome research

Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

Fri Apr 06 00:00:00 UTC 2018

A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1α stability.

Nature communications

Liu J,Wang Y,Song L,Zeng L,Yi W,Liu T,Chen H,Wang M,Ju Z,Cong YS

Fri Jan 27 00:00:00 UTC 2017

A critical role of DDRGK1 in endoplasmic reticulum homoeostasis via regulation of IRE1α stability.

Nature communications

Liu J,Wang Y,Song L,Zeng L,Yi W,Liu T,Chen H,Wang M,Ju Z,Cong YS

Fri Jan 27 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: Dashurin; DDRGK domain-containing protein 1; UFM1-binding and PCI domain-containing protein 1; UFM1-binding protein 1 containing a PCI domain

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Gene Aliases: 1110001I20Rik; 2600009E05Rik; AI326138; C20orf116; DDRGK1; dJ1187M17.3; UFBP1

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UniProt ID: (Human) Q96HY6, (Mouse) Q80WW9

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Entrez Gene ID: (Human) 65992, (Mouse) 77006

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Function(s)

protein binding ubiquitin-like protein ligase binding

Process(es)

regulation of intracellular estrogen receptor signaling pathway response to endoplasmic reticulum stress positive regulation of NF-kappaB transcription factor activity positive regulation of proteasomal protein catabolic process positive regulation of I-kappaB phosphorylation protein K69-linked ufmylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DDRGK1 Polyclonal Antibody

DDRGK1 Antibody (21445-1-AP) in ICC/IF

Product Details

21445-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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