Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
DDX1 Monoclonal Antibody (2D4)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 7
DDX1 Antibody (MA5-49263) in IHC (P)
Immunohistochemistry analysis of DDX1 in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with DDX1 Monoclonal antibody (Product # MA5-49263) using a dilution of 2 μg/mL overnight at 4°C. Biotinylated goat anti-m... View More
Product Details
MA5-49263
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2D4
Immunogen
E.coli-derived human DDX1 recombinant protein (Position: K562-F740).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074921
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human CACO-2 whole cell, human U20S whole cell, human HEK293 whole cell, human U87 whole cell, human HELA whole cell, human A549 whole cell, rat kidney tissue, mouse kidney tissue. IHC: human gastric cancer tissue, human pancreatic cancer tissue, rat brain tissue, human esophageal squamous carcinoma tissue. Flow: MCF-7 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
Gene Aliases: AA409185; DBP-RB; DDX1; UKVH5d
UniProt ID: (Human) Q92499, (Rat) Q641Y8, (Mouse) Q91VR5
Entrez Gene ID: (Human) 1653, (Rat) 84474, (Mouse) 104721
DNA binding
chromatin binding
transcription cofactor activity
RNA helicase activity
double-stranded RNA binding
ATP-dependent RNA helicase activity
nuclease activity
exonuclease activity
protein binding
ATP binding
ATP-dependent helicase activity
poly(A) binding
DNA/RNA helicase activity
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
helicase activity
hydrolase activity
spliceosomal complex assembly
double-strand break repair
transcription, DNA-templated
regulation of transcription, DNA-templated
tRNA splicing, via endonucleolytic cleavage and ligation
regulation of translational initiation
multicellular organism development
response to virus
RNA secondary structure unwinding
DNA duplex unwinding
response to exogenous dsRNA
nucleic acid phosphodiester bond hydrolysis
protein localization to cytoplasmic stress granule
mRNA processing
tRNA processing
regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support