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Invitrogen
DDX1 Monoclonal Antibody (3E5E2)
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FIGURE: 1 / 8
DDX1 Antibody (MA5-31712) in ICC/IF
Immunocytochemistry analysis of DDX1 in Hela cells (green). Sample was incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
Product Details
MA5-31712
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3E5E2
Immunogen
Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787336
Product Specific Information
MA5-31712 has been tested in indirect ELISA.
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
Gene Aliases: AA409185; DBP-RB; DDX1; UKVH5d
UniProt ID: (Human) Q92499, (Mouse) Q91VR5
Entrez Gene ID: (Human) 1653, (Mouse) 104721
DNA binding
chromatin binding
transcription cofactor activity
RNA helicase activity
double-stranded RNA binding
ATP-dependent RNA helicase activity
nuclease activity
exonuclease activity
protein binding
ATP binding
ATP-dependent helicase activity
poly(A) binding
DNA/RNA helicase activity
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
helicase activity
hydrolase activity
spliceosomal complex assembly
double-strand break repair
transcription, DNA-templated
regulation of transcription, DNA-templated
tRNA splicing, via endonucleolytic cleavage and ligation
regulation of translational initiation
multicellular organism development
response to virus
RNA secondary structure unwinding
DNA duplex unwinding
response to exogenous dsRNA
nucleic acid phosphodiester bond hydrolysis
protein localization to cytoplasmic stress granule
mRNA processing
tRNA processing
regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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