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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • DDX23 Antibodies

          Invitrogen

          DDX23 Polyclonal Antibody

          View all (9) DDX23 antibodies

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          Datasheet
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          Datasheet
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          Cite DDX23 Polyclonal Antibody

          • Antibody Testing Data (4)
          DDX23 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          DDX23 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          DDX23 Antibody (PA5-30312) in ICC/IF

          DDX23 Polyclonal Antibody detects DDX23 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDX23 protein stained by DDX23 Polyclonal Antibody (Product # PA5-30312) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          DDX23 Antibody in Immunocytochemistry (ICC/IF)
          DDX23 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DDX23 Antibody in Western Blot (WB)
          DDX23 Antibody in Western Blot (WB)

          Product Details

          PA5-30312

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human DDX23. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2547786

          Product Specific Information

          Recommended positive controls: Jurkat, NIH-3T3, JC.

          Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (91%), Rhesus Monkey (100%), Bovine (100%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 100 kDa U5 snRNP-specific protein; DEAD (Asp-Glu-Ala-Asp) box polypeptide 23; DEAD box protein 23; Probable ATP-dependent RNA helicase DDX23; PRP28 homolog; PRP28 homolog, yeast; PRP28p homolog; U5 snRNP 100 kD protein; U5-100kD

          View more View less

          Gene Aliases: 3110082M05Rik; 4921506D17Rik; DDX23; prp28; PRPF28; SNRNP100; U5-100K; U5-100KD

          View more View less

          UniProt ID: (Human) Q9BUQ8

          View more View less

          Entrez Gene ID: (Human) 9416, (Mouse) 74351

          View more View less

          Function(s)
          ATP-dependent RNA helicase activity protein binding ATP binding ATP-dependent helicase activity poly(A) RNA binding
          Process(es)
          cis assembly of pre-catalytic spliceosome RNA splicing, via transesterification reactions mRNA splicing, via spliceosome RNA splicing RNA secondary structure unwinding
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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