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Invitrogen
DDX23 Polyclonal Antibody
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FIGURE: 1 / 5
DDX23 Antibody (PA5-34843) in ICC/IF
Immunocytochemistry (Confocal immunofluorescence) of DDX23 in paraformaldehyde-fixed HeLa. Samples were incubated with DDX23 polyclonal antibody (Product # PA5-34843) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,000.
Product Details
PA5-34843
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human DDX23. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2552195
Product Specific Information
Recommended positive controls: HeLa, NIH-3T3, JC.
Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (86%), Rhesus Monkey (100%), Bovine (100%).
IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 100 kDa U5 snRNP-specific protein; DEAD (Asp-Glu-Ala-Asp) box polypeptide 23; DEAD box protein 23; Probable ATP-dependent RNA helicase DDX23; PRP28 homolog; PRP28 homolog, yeast; PRP28p homolog; U5 snRNP 100 kD protein; U5-100kD
Gene Aliases: 3110082M05Rik; 4921506D17Rik; DDX23; prp28; PRPF28; SNRNP100; U5-100K; U5-100KD
UniProt ID: (Human) Q9BUQ8
Entrez Gene ID: (Human) 9416, (Mouse) 74351
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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