Proteintech

DDX3Y Polyclonal Antibody

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Datasheet
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Questions & Answers

Product Details

14041-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500
-

Immunocytochemistry (ICC/IF)

1:10-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DDX3Y Fusion Protein Ag5110 (311-660 aa encoded by BC034942)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Polyclonal anti-DDX3Y antibodies (14041-1-AP) are produced by immunizing animals with C-terminus of DDX3Y and detects a 73 kDa band. As DDX3Y has a structural homolog with DDX3X, the antibody can recognize both.

Immunogen sequence: LERGCHLLV ATPGRLVDMM ERGKIGLDFC KYLVLDEADR MLDMGFEPQI RRIVEQDTMP PKGVRHTMMF SATFPKEIQM LARDFLDEYI FLAVGRVGST SENITQKVVW VEDLDKRSFL LDILGATGSD SLTLVFVETK KGADSLEDFL YHEGYACTSI HGDRSQRDRE EALHQFRSGK SPILVATAVA ARGLDISNVR HVINFDLPSD IEEYVHRIGR TGRVGNLGLA TSFFNEKNMN ITKDLLDLLV EAKQEVPSWL ENMAYEHHYK GGSRGRSKSN RFSGGFGARD YRQSSGSSSS GFGASRGSSS RSGGGGYGNS RGFGGGGYGG FYNSDGYGGN YNSQGVDWWG N (311-660 aa encoded by BC034942)

Target Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. DDX3Y is one member of DEAD box RNA helicases, encode by the gene locate on the chromosome Y in the azoospermia factor A region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent RNA helicase DDX3Y; D1Pas1 related sequence 1; D1Pas1-related sequence 1; DEAD (Asp-Glu-Ala-Asp) box helicase 3, Y-linked; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide, Y chromosome; DEAD box protein 3, Y-chromosomal; DEAD-box RNA helicase DEAD2; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, Y chromosome; mDEAD2; uncharacterized protein LOC364073

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Gene Aliases: 8030469F12Rik; D1Pas1-rs1; DBY; DDX3Y; Dead2; RGD1309586

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UniProt ID: (Human) O15523, (Mouse) Q62095

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Entrez Gene ID: (Human) 8653, (Rat) 364073, (Mouse) 26900

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Function(s)
DNA binding RNA binding ATP-dependent RNA helicase activity ATP binding nucleic acid binding GTPase activity ATP-dependent DNA helicase activity helicase activity transcription factor binding poly(A) binding eukaryotic initiation factor 4E binding ATPase activity nucleoside-triphosphatase activity translation initiation factor binding RNA stem-loop binding ribosomal small subunit binding CTPase activity poly(A) RNA binding mRNA 5'-UTR binding nucleotide binding hydrolase activity
Process(es)
chromosome segregation metabolic process extrinsic apoptotic signaling pathway via death domain receptors response to virus RNA secondary structure unwinding positive regulation of gene expression negative regulation of translation positive regulation of cell growth negative regulation of cell growth negative regulation of protein complex assembly DNA duplex unwinding stress granule assembly intracellular signal transduction mature ribosome assembly positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of viral genome replication innate immune response positive regulation of translation positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation cellular response to arsenic-containing substance cellular response to osmotic stress intrinsic apoptotic signaling pathway positive regulation of G1/S transition of mitotic cell cycle negative regulation of intrinsic apoptotic signaling pathway translational initiation regulation of gene expression
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