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Invitrogen
DDX5 Monoclonal Antibody (Ab204), eBioscience™
FIGURE: 1 / 1
DDX5 Antibody (14-6005-82)
Western blot analysis of siRNA-treated A549 cells. Clone Ab204 detects the ~68 kDa band relating to the DDX5 protein in the lysate of untreated A549 cells (lane 3). As expected, the bands representing the lysates of DDX5-specific siRNA treated cells (lanes 1 and 2) are less intense consistent with the lower amount of DDX5 protein in cells. Details: A549 cells were either transfected with DDX5 gene Silencer ... View More
Product Details
14-6005-82
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
Ab204
Immunogen
Purified T antigen-related protein from Ad2+ ND2 virus-infected HeLa cells
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802195
Product Specific Information
Description: This Ab204 monoclonal antibody detects human DDX5 protein also known as DEAD box protein 5 and RNA helicase p68. This antibody has been reported to cross-react with mouse and rat orthologs of DDX5 protein.
Applications Reported: This Ab204 antibody has been reported for use in western blot and Immunocytochemistry. (Fluorochrome-conjugated Ab204 is recommended for use in flow cytometry.)
Applications Tested: This Ab204 antibody has been tested by western blot of mouse lymph node cells. This may be used at less than or equal to 4.0 µg/mL.
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DKFZp434E109; DKFZp686J01190; MGC118083; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68; RP23-247J12.4
Gene Aliases: DDX5; G17P1; HELR; HLR1; HUMP68; p68
UniProt ID: (Human) P17844
Entrez Gene ID: (Human) 1655
negative regulation of transcription from RNA polymerase II promoter
regulation of alternative mRNA splicing, via spliceosome
mRNA splicing, via spliceosome
mRNA transcription
RNA secondary structure unwinding
cell growth
positive regulation of intracellular estrogen receptor signaling pathway
positive regulation of DNA damage response, signal transduction by p53 class mediator
regulation of viral genome replication
regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
regulation of androgen receptor signaling pathway
intrinsic apoptotic signaling pathway by p53 class mediator
regulation of skeletal muscle cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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