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Invitrogen
DDX5 Monoclonal Antibody (Ab204), Alexa Fluor™ 488, eBioscience™
FIGURE: 1 / 3
DDX5 Antibody (53-6005-42) in ICC/IF
Immunofluorescent analysis of DDX5 (green) in AF549 cells (45% confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at 37°C, and blocked with 10% Normal Mouse Serum in PBS for 1 hour at room temperature. Cells were then stained with DDX5 Monoclonal Antibody, conjugated to Alexa Fluor 488 at a 1:20 dilution or 0.025 µg/mL Mouse IgG1 isotype control conjugated to Alexa Fluor 488 at 4°C overnight in blocking buffer. Cell nuclei were stained with NucB... View More
Product Details
53-6005-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Ab204
Immunogen
Purified T antigen-related protein from Ad2+ ND2 virus-infected HeLa cells
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802337
Product Specific Information
Description: This Ab204 monoclonal antibody detects human DDX5 protein also known as DEAD box protein 5 and RNA helicase p68. This antibody has been reported to cross-react with mouse and rat orthologs of DDX5 protein.
Applications Reported: This Ab204 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This Ab204 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Applications Reported: This Ab204 antibody has been reported for use in immunocytochemical staining.
Applications Tested: This Ab204 antibody has been pre-diluted and tested by immunocytochemical staining on cultured A549 cells using PFA fixation. This may be used at 2.5 µL (0.025 µg) per ml.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); DKFZp434E109; DKFZp686J01190; MGC118083; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68; RP23-247J12.4
Gene Aliases: DDX5; G17P1; HELR; HLR1; HUMP68; p68
UniProt ID: (Human) P17844
Entrez Gene ID: (Human) 1655
negative regulation of transcription from RNA polymerase II promoter
regulation of alternative mRNA splicing, via spliceosome
mRNA splicing, via spliceosome
mRNA transcription
RNA secondary structure unwinding
cell growth
positive regulation of intracellular estrogen receptor signaling pathway
positive regulation of DNA damage response, signal transduction by p53 class mediator
regulation of viral genome replication
regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
regulation of androgen receptor signaling pathway
intrinsic apoptotic signaling pathway by p53 class mediator
regulation of skeletal muscle cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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