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DDX52 Antibody (A303-053A) in IP
Detection of human DDX52 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX52 antibody (Product # A303-053A) used for IP at 6 µg/mg lysate. DDX52 was also immunoprecipitated by rabbit anti-DDX52 antibody A303-054A, which recognizes a downstream epitope. For blotting immunoprecipitated DDX52, A303-054A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Product Details
A303-053A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 499 and 549 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 52.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and Bovine
Target Information
The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase ROK1-like; DEAD (Asp-Glu-Ala-Asp) box polypeptide 52; DEAD box protein 52; Probable ATP-dependent RNA helicase DDX52
Gene Aliases: DDX52; HUSSY-19; HUSSY19; ROK1
UniProt ID: (Human) Q9Y2R4
Entrez Gene ID: (Human) 11056
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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