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Invitrogen
DDX6 Monoclonal Antibody (8G6)
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FIGURE: 1 / 4
DDX6 Antibody (MA5-49268) in ICC/IF
Immunocytochemistry analysis of DDX6 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in DDX6 Monoclonal antibody (Product # MA5-49268) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for ... View More
Product Details
MA5-49268
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
8G6
Immunogen
E.coli-derived human DDX6 recombinant protein (Position: Q56-P483).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074926
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human Sw620 whole cell, human Raji whole cell, human THP-1 whole cell, human Jurkat whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell. ICC/IF: MCF-7 cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase p54; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 6; DEAD (Asp-Glu-Ala-Asp) box helicase 6; DEAD (Asp-Glu-Ala-Asp) box polypeptide 6; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 6; DEAD box protein 6; DEAD box-6; DEAD-box protein 6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD); Oncogene RCK; Oncogene RCK homolog; Probable ATP-dependent RNA helicase DDX6
Gene Aliases: 1110001P04Rik; DDX6; E230023J21Rik; HLR2; mRCK/P54; P54; RCK; RGD1564560
UniProt ID: (Human) P26196, (Mouse) P54823
Entrez Gene ID: (Human) 1656, (Mouse) 13209, (Rat) 500988
regulation of translation
RNA secondary structure unwinding
viral RNA genome packaging
stem cell population maintenance
cytoplasmic mRNA processing body assembly
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
negative regulation of neuron differentiation
cell-cell adhesion
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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