Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
DEDD Antibody (PA5-22193) in ICC/IF
Immunocytochemistry (Confocal immunofluorescence) of DEDD in paraformaldehyde-fixed HeLa. Samples were incubated with DEDD polyclonal antibody (Product # PA5-22193) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,500.
Product Details
PA5-22193
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human DEDD. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11156014
Product Specific Information
Recommended positive controls: mouse leukocyte treated with CFA.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DEDD encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Death effector domain-containing protein; Death effector domain-containing testicular molecule; DEDPro1; FLDED-1; tumor necrosis factor (ligand) superfamily, member 19
Gene Aliases: CASP8IP1; DEDD; DEDD1; DEDPRO1; DEFT; FLDED1; KE05
UniProt ID: (Human) O75618, (Mouse) Q9Z1L3
Entrez Gene ID: (Human) 9191, (Mouse) 21945
transcription, DNA-templated
regulation of transcription, DNA-templated
apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of protein catabolic process
regulation of apoptotic process
decidualization
negative regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
spermatogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support